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Fastbreak specifications

Information


Unique identifier OMICS_23252
Name Fastbreak
Interface Web user interface
Restrictions to use None
Input data A DNA sequence.
Output data A graphical and numerical result.
Programming languages Javascript, Python
Computer skills Basic
Stability Stable
Maintained Yes

Maintainer


  • person_outline Ilya Shmulevich <>

Additional information


http://fastbreak.systemsbiology.net/

Information


Unique identifier OMICS_23252
Name Fastbreak
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Input data A DNA sequence.
Input format SAM,BAM
Output data A graphical and numerical result.
Operating system Unix/Linux, Mac OS, Windows
Programming languages Javascript, Python
Computer skills Medium
Stability Stable
Maintained Yes

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Maintainer


  • person_outline Ilya Shmulevich <>

Additional information


http://fastbreak.systemsbiology.net/

Publication for Fastbreak

Fastbreak in publications

 (26)
PMCID: 5874916
PMID: 29588400
DOI: 10.1128/mBio.00316-18

[…] 0.4 to 0.8, expression was induced with 400 µm isopropyl-β-d-thiogalactopyranoside (iptg; sigma-aldrich). the cells were harvested after 5 h of induction, and the cell pellets were lysed in 1× fastbreak cell lysis reagent (promega). the 6×his-tagged wt rtp51/p66 heterodimer was purified with magnehis ni-particles (promega). after binding with rt, the beads were washed in wash buffer (50 mm […]

PMCID: 5763143
PMID: 29375542
DOI: 10.3389/fimmu.2017.01759

[…] sucrose shock and purified by immobilized metal affinity chromatography as previously described (). for small-scale expression screening, 5 ml overnight cultures were grown as above, lysed using fastbreak™ reagent (promega, madison, wi, usa) and sdabs purified using pureproteome™ nickel magnetic beads (emd millipore, billerica, ma, usa). expression yields from 5 ml cultures were determined […]

PMCID: 5595907
PMID: 28900282
DOI: 10.1038/s41598-017-11796-8

[…] β-d-1-thiogalactopyranoside (iptg) at 30 °c for 3 hrs. the cells were then harvested by brief centrifugation and resuspended in a solution containing 50 mm sodium phosphate (ph 8.0), 0.3 m nacl, 1× fastbreak reagent (promega, wi, usa), 100 μg/ml lysozyme, and 100 μg/ml dnase i. to remove cell debris, the cell lysate was centrifuged at 20,400 g for 10 min and the supernatants were collected. […]

PMCID: 5221617
PMID: 28035045
DOI: 10.1091/mbc.E16-06-0451

[…] incubation, isopropyl β-d-1-thiogalactopyranoside (sigma-aldrich) was added (final concentration 0.25 mm), and the culture was incubated at 30°c for 4 h. after the incubation, cells were lysed using fastbreak cell lysis reagent (promega) according to the manufacturer’s instructions. the cell lysate was then centrifuged and the supernatant collected. the presence of gst-gfp-p4m was confirmed […]

PMCID: 5059447
PMID: 27698405
DOI: 10.1038/ncomms13014

[…] acid, ph 10.5, 500 mm nacl, 30 mm imidazole, ph 10.5 and stored at −20 °c. the bacterial cells were lysed by two repetitive freeze-thaw cycles in the presence of 20 mg lysozyme, 0.5 mg of dnasei and fastbreak cell lysis buffer (1x; promega). lysates were centrifuged at 15,000 r.p.m. for 30 min at 4 °c, and the supernatant filtered through a 0.45 μm low protein-binding filter and applied to a 5 ml […]


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Fastbreak institution(s)
Institute for System Biology, Seattle, WA, USA; Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Amsterdam, Netherlands
Fastbreak funding source(s)
Supported by National Cancer Institute the Fund for Scientific Research Flanders under Krediet aan Navorsers 1.5.207.08 and 1.5.118.09.N.00; the Hunagarian Research Fund under grants No. PD79183, and No. K75460; the Children Cancer Fund Ghent under the grant No. G.0198.08; and the Methusalem program BOF08/01M01108.

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