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FASTmC specifications


Unique identifier OMICS_10589
Interface Web user interface
Restrictions to use None
Input format FASTQ
Computer skills Basic
Stability Stable
Maintained Yes


  • person_outline Robert Schmitz

Publication for FASTmC

FASTmC citations


Epigenomic diversification within the genus Lupinus

PLoS One
PMCID: 5480990
PMID: 28640886
DOI: 10.1371/journal.pone.0179821

[…] DNA was isolated from leaf tissue and MethylC-seq libraries were prepared for each species as was previously described [, ]. As no sequenced and assembled genome exists for Lupinus spp., we used FASTmC, which is a non-reference-based approach to estimate the levels of DNA methylation in the CG, CHG (H = A|C|T) and CHH sequence contexts []. Briefly, linear models were created for each sequence […]


Evolution of DNA Methylation across Insects

Mol Biol Evol
PMCID: 5400375
PMID: 28025279
DOI: 10.1093/molbev/msw264

[…] ast squares (PGLS) was used to correlate continuously categorized estimates of DNA methylation generated through WGBS and alignment to reference assemblies (methylpy), or nonreferenced based methods (FASTmC) to discretely coded social traits (eusocial, subsocial, communal, and solitary). BEAST 2 () was used to estimate a multilocus coalescent tree, which was used to control for relatedness of spec […]


A Comparative Analysis of 5 Azacytidine and Zebularine Induced DNA Demethylation

PMCID: 5015934
PMID: 27402357
DOI: 10.1534/g3.116.030262

[…] sed to remove low quality reads using Methylpy (). A second replicate of control samples and seedlings treated with 100 µM of AZA or ZEB were lightly sequenced (Table S2) and run through the web tool FASTmC, a tool for genome-wide estimation of DNA methylation levels ().Genome-wide methylation levels from deeply sequenced samples were calculated using weighted methylation (). BedTools () was used […]

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FASTmC institution(s)
Department of Genetics, University of Georgia, Athens, GA, USA
FASTmC funding source(s)
The National Science Foundation (MCB-1339194); the National Institutes of Health (R00GM100000)

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