Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 13
Release date: Oct 23 2013
Last update date: Apr 18 2017
Access: Public
Diseases: Stomach Diseases
Dataset link Expression profiling of Troy positive gastric cells

Experimental Protocol


An EGFP-ires-CreERT2 cassette was introduced into the transcriptional start side of Tnfrsf19 (Troy), resulting in the expression of EGFP and CreERT2 under the control of endogenous Troy-regulatory sequences. Troy is expressed in basally located chief and parietal cells. Using FACS, Troy positive chief and parietal cells can be isolated separately. Expression profiling was performed on these two cell types: three arrays of sorted chief cells, two arrays of sorted parietal cells. As a reference, two arrays of separately isolated whole gastric corpus glands were added. In addition, single sorted Troy positive chief cells can be placed in culture, forming 3D cultures called organoids. Three arrays were generated from different organoid cultures, one time in normal medium containing the factors EGF, Gastrin, FGF10, Noggin, Wnt3a and Rspondin (EGFNWR medium) and one time in medium containing only EGF and Rspondin (ER medium).

Repositories


GEO

GSE44060

ArrayExpress

E-GEOD-44060

BioProject

PRJNA188502

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Contact


Daniel Stange
Daniel E. Stange