Dataset features

Specifications


Application: RNA-seq analysis, sRNA-seq analysis, BS-seq analysis
Number of samples: 37
Release date: Jul 20 2017
Last update date: Oct 24 2018
Access: Public
Genes: Pld6, Dnmt3l, DNMT3L, PLD6
Taxon: Homo sapiens, Mus musculus
Dataset link The effect of the Pld6 and Dnmt3l mutations on DNA methylation and expression of retrotransposons in mouse male germ cells

Experimental Protocol


To study the effect of the Pld6 and Dnmt3l mutations on retrotransposon regulation, we determined the DNA methylation levels in the mutant spermatogonia and the RNA expression levels of retrotransposons in the mutant testes at P0 (containing prospermatogonia) and mutant leptotene/zygotene spermatocytes using bowtie2 (both are available as supplementary tables in the original article). We also determined genome-wide DNA methylation map (using bismark) and the levels of gene expression (using Tophat and Cuffdiff), which are available as processed files here. We also analyzed small RNAs in spermatogonia, spermatocytes and round spermatids to analyze the population of piRNAs in these cells.

Repositories


GEO

GSE70891

BioProject

PRJNA289866

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Contact


Kota Inoue

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