|Number of samples:||115|
|Release date:||Jan 17 2013|
|Last update date:||Nov 21 2018|
|Dataset link||Latent enhancers unveiled by stimulation expand and adapt the available cis-regulatory repertoire (ChIP-seq)|
Chromatin immunoprecipitations of H3 lysine 4 mono-methylated, H3 lysine 27 acetylated, H3 lysine 4 tri-methylated, the transcription factors PU.1, Stat1, Stat6 and JunB and the total RNA polymerase II followed by multiparallel sequencing performed in murine bone marrow-derived macrophages (BMDMs). Experiments were carried out in untreated cells as well as in cells treated for 4hrs (lipopolysaccharide (LPS), IFNg, IL4, TNFa, TGFb1, IL1b, MALP2, CpG) and 24hrs (LPS). Same experiments were carried out in Stat1 KO (BMDM stimulated with IFNg) and Stat6 KO (BMDM stimulated with IL4) backgrounds. In case of IL4 an independent time-course was performed considering 15', 30', 1hrs, 2hrs and 4hrs after treatment while for IFNg we considered 30', 1hrs and 2hrs after treatment. For washout experiments, IP against H3 lysine 4 mono-methylated was performed 24hrs and 72hrs after stimulation as well as 48 hours after washout of the stimulus (24hrs).
Iros Giacomo Barozzi