Computational protocol: A Novel, Diffusely Infiltrative Xenograft Model of Human Anaplastic Oligodendroglioma with Mutations in FUBP1, CIC, and IDH1

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Protocol publication

[…] Agilent SureSelect target enrichment kit (SureSelect Human All Exon 50 Mb kit, Agilent) was used for exome library construction according to the manufacturer's protocol. Captured DNA libraries were sequenced on the Illumina HiSeq2000 platform. Reads were aligned to the human reference genome (hg19) with BWA. Duplicate tags were removed. Point mutations, and insertions and deletions (INDELs) were identified using Samtools (version 0.1.18) with default parameters. Somatic variations were then validated in IGV browser 2.1.22. […]

Pipeline specifications

Software tools BWA, SAMtools, IGV
Application WES analysis
Organisms Mus musculus, Homo sapiens
Diseases Glioma, Neoplasms, Oligodendroglioma, X-Linked Combined Immunodeficiency Diseases