Computational protocol: A novel method to study contact inhibition of locomotion using micropatterned substrates

Similar protocols

Protocol publication

[…] Time-lapse movies and cell tracking of migrating NC cells were performed as previously described (). Tracks were generated using ImageJ Manual Tracking plug-in and Imaris. “Run and tumble” was analyzed as described (). Cell protrusions were analyzed as described (). In brief, cell protrusions were defined by the positive difference in the area of a cell between two consecutive frames. Retraction was defined as the negative difference in the area of a cell between two consecutive frames. Protrusion and retraction areas were analyzed by using the ImageJ Analyze Particle plug-in. Distance between nuclei was calculated on ImageJ. Velocity and acceleration were extracted from Imaris tracks. Statistical analyses were performed using Excel software and Prism4. […]

Pipeline specifications

Software tools ImageJ, Imaris
Application Microscopic phenotype analysis
Diseases Neoplasms