Computational protocol: Evaluation of a Hybrid Approach Using UBLAST and BLASTX for Metagenomic Sequences Annotation of Specific Functional Genes

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Protocol publication

[…] UBLAST is one of the tools in USEARCH (version 7.0). The free version of 32-bit USEARCH was downloaded from Search acceleration setting “-accel” of UBLAST was tested using the value of 0.5, 0.8 and 1. Default termination options (“-maxaccepts” and “-maxrejects”) were used in all of the tests. Potential matched sequences were extracted from the metagenomic dataset using Perl. Version of BLASTX program is 2.2.28+. Commands used in the tests were listed in . […]

Pipeline specifications

Software tools USEARCH, BLASTX
Application Metagenomic sequencing analysis
Chemicals Arsenic, Nitrogen