Computational protocol: Suppression of ERK signalling abolishes primitive endoderm formation but does not promote pluripotency in rabbit embryo

Similar protocols

Protocol publication

[…] Embryos were placed on a glass-bottom dish (MatTek) and visualised using a Nikon R1 confocal microscope. Analysis of images was performed using IMARIS software (Bitplane AG). For cell number count, nuclei were identified using the ‘spot’ option with an estimated diameter of 7-10 μm. The number of nuclei identified by IMARIS was confirmed manually. 3D confocal images were created by maximum intensity projection using the IMARIS software (Bitplane AG) ‘volume’ option. [...] Analysis was performed using IBM SPSS Statistics 22.0 software. Before computing, all data were subjected to Kolmogorov–Smirnov tests for normal distribution. Statistical differences in gene expression level between developmental stages and differences in cell number between groups were calculated using Kruskal–Wallis test and nonparametric Mann–Whitney test. Differences in percentage of cells between groups was analysed using Z-tests. P<0.05 was considered statistically significant. […]

Pipeline specifications

Software tools Imaris, SPSS
Applications Miscellaneous, Laser scanning microscopy
Organisms Oryctolagus cuniculus, Homo sapiens, Mus musculus