|Application:||miRNA array analysis|
|Number of samples:||6|
|Release date:||Oct 15 2014|
|Last update date:||Jan 7 2015|
|Dataset link||Dynamics of intercellular and exosomal miRNAs in hypoxia-resistant RPMI8226 cells|
RPMI8226 cells were cultured for 24 hours under hypoxic conditions (1% O2) or normoxic conditions (20% O2). The exosome fraction was obtained from culture medium using Exoquick Exosome Precipitation Solution (System Biosciences, Mountain View, CA, USA). Isolation of cellular and exosomal miRNAs was performed using the miRNsasy kit (Qiagen). The expression profile of miRNAs was determined using the Human Taqman miRNA Arrays A (Applied Biosystems). RNU6B and a spike control (ath-miR159) were used as an invariant control for the cell and exosome, respectively. QRT-PCR was carried out on an Applied Biosystems 7900HT thermal cycler using the manufacturerâs recommended program. Finally, all the raw data from each array was run on Data Assist Software ver.3.1 (Applied Biosystems).