Dataset features

Specifications


Application: miRNA array analysis
Number of samples: 6
Release date: Oct 15 2014
Last update date: Jan 7 2015
Access: Public
Diseases: Multiple Myeloma
Dataset link Dynamics of intercellular and exosomal miRNAs in hypoxia-resistant RPMI8226 cells

Experimental Protocol


RPMI8226 cells were cultured for 24 hours under hypoxic conditions (1% O2) or normoxic conditions (20% O2). The exosome fraction was obtained from culture medium using Exoquick Exosome Precipitation Solution (System Biosciences, Mountain View, CA, USA). Isolation of cellular and exosomal miRNAs was performed using the miRNsasy kit (Qiagen). The expression profile of miRNAs was determined using the Human Taqman miRNA Arrays A (Applied Biosystems). RNU6B and a spike control (ath-miR159) were used as an invariant control for the cell and exosome, respectively. QRT-PCR was carried out on an Applied Biosystems 7900HT thermal cycler using the manufacturer’s recommended program. Finally, all the raw data from each array was run on Data Assist Software ver.3.1 (Applied Biosystems).

Repositories


GEO

GSE48909

ArrayExpress

E-GEOD-48909

BioProject

PRJNA212526

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Contact


Tomohiro Umezu