Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 10
Release date: Jul 1 2011
Last update date: Mar 22 2012
Access: Public
Diseases: Myositis
Chemicals: Amino Acids
Dataset link Comparative genomics and transcriptomics of Propionibacterium acnes

Experimental Protocol


Microarray experiments were performed as dual-color hybridizations. In order to compensate specific effects of the dyes and to ensure statistically relevant data analysis, a color-swap dye-reversal was performed. Two different labeling methods were applied. RNA labeling was performed with the two color Quick Amp Labeling Kit (Agilent Technologies) using FullSpectrum MultiStart Primer for T7 IVT RNA Amplification (BioCat GmbH, Heidelberg, Germany) as random T7 labeling. Alternatively, the total RNA samples were amplified with the TransPlex Whole Transcriptome Amplification Kit (Sigma-Aldrich, Munich, Germany) and labeled with BioPrime Plus Array CGH Indirect Genomic Labeling System (Invitrogen, Karlsruhe, Germany) as WTA BioPrime indirect. Both methods were compared and combined for final data analysis.

Repositories


GEO

GSE26738

ArrayExpress

E-GEOD-26738

BioProject

PRJNA136323

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Contact


Hans-Joachim Mollenkopf

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