Fluorescence correlation spectroscopy data analysis | Laser scanning microscopy

Assists users for the interactive and automated processing of fluorescence auto- and cross-correlation spectroscopy (FCS/FCCS) data. Fluctuation Analyzer is a software that can read raw data, such as channel photon streams, from various commercial suppliers of FCS/FCCS data acquisition equipment. It also permits users to organize data in processing sessions by file-based management, calculate temporal auto- and cross-correlation functions, and fit the data with appropriate model functions before saving the results.

Allows users to statistically analyze and summarize large-scale fluorescence auto- and cross-correlation spectroscopy (FCS/FCCS) data. Fluctuation Statistics can read results files from the accompanying Fluctuation Analyzer software, applies required calibrations, normalizations and corrections and creates HTML-formatted report sheets. It also permits users to interactively explore the biophysical parameters extracted from larger numbers of cellular FCS/FCCS measurements.

Provides a standardized environment for the analyses of correlation data. PyCorrFit is delivered with several integrated model functions, covering a wide range of applications in standard confocal fluorescence correlation spectroscopy (FCS). It also contains equations dealing with different stimulation geometries. This method was developed to support many data file formats and to feature a set of tools specialized in data evaluation.

Evaluates image stacks acquired in imaging fluorescence correlation spectroscopy (FCS). ImFCS is a data analysis tool for camera based FCS that calculates a variety of spatiotemporal auto- and cross-correlations and differences between spatial forward and backward cross-correlations. It can facilitate the analysis imaging FCS data for the community interested in the dynamic behavior of molecules in model and living systems.

Extracts fluctuating fluorescence from measurements. PyScanFCS serves especially for perpendicular line scanning fluorescence correlation spectroscopy.