Fluorescence correlation spectroscopy data analysis | Laser scanning microscopy
Fluorescence correlation spectroscopy (FCS) is used to study the movements and the interactions of biomolecules at extremely dilute concentrations, yielding results with good spatial and temporal resolutions. Using a number of technical developments, FCS has become a versatile technique that can be used to study a variety of sample types and can be advantageously combined with other methods. Unlike other fluorescence-based techniques, the analysis of FCS data is not based on the average intensity of the fluorescence emission but examines the minute intensity fluctuations caused by spontaneous deviations from the mean at thermal equilibrium.
Represents an integrated solution for data analysis and acquisition. SymPhoTime 64 permits users to target principally the results rather than the data processing. The interface of this tool simplifies the utilization by the user for an individual analysis or measurement process. Different data acquisition modes including interleaved trains of excitation and stimulated emission depletion (STED) laser pulses are integrated in a graphical user interface (GUI).
Uses for fluorescence image analysis, visualization, simulation, and acquisition. SimFCS is a scientific software for data form spectroscopy and fluorescence microscopy. It can be applied to Fluorescence Correlation Spectroscopy, Fluorescence Lifetime Imaging, Pair correlation function, Image Mean Square Displacement, Single Particle and Modulation Tracking, Raster and Spatio-temporal Image Correlation Spectroscopy and many other.
Assists users with data evaluation. QuickFit is an open-source software for flow cytometry standard (FCS) and imagingFCS (imFCS) measurement. It also provides microscope characterization plugins such as selective plane illumination microscopy (SPIM) lightsheet characterization, camera calibration or colocalization analysis. It also includes a customizable user interface and a spectra viewer with comprehensive set of spectra.
Evaluates image stacks acquired in imaging fluorescence correlation spectroscopy (FCS). ImFCS is a data analysis tool for camera based FCS that calculates a variety of spatiotemporal auto- and cross-correlations and differences between spatial forward and backward cross-correlations. It can facilitate the analysis imaging FCS data for the community interested in the dynamic behavior of molecules in model and living systems.
Provides a standardized environment for the analyses of correlation data. PyCorrFit is delivered with several integrated model functions, covering a wide range of applications in standard confocal fluorescence correlation spectroscopy (FCS). It also contains equations dealing with different stimulation geometries. This method was developed to support many data file formats and to feature a set of tools specialized in data evaluation.
Provides a visual basic for application (VBA) macro to be used with Zeiss confocal microscopes. MyPiC allows autofocus based on reflection and fluorescence multi-location time series and fluorescence-based tracking using the center of mass of the fluorescence signal. It also permits flexible combination of several independent Z-stack and channel settings and of imaging with fluorescence correlation spectroscopy (FCS) experiments.