Fluorescence correlation spectroscopy data analysis | Laser scanning microscopy

Assists users for the interactive and automated processing of fluorescence auto- and cross-correlation spectroscopy (FCS/FCCS) data. Fluctuation Analyzer is a software that can read raw data, such as channel photon streams, from various commercial suppliers of FCS/FCCS data acquisition equipment. It also permits users to organize data in processing sessions by file-based management, calculate temporal auto- and cross-correlation functions, and fit the data with appropriate model functions before saving the results.

Allows users to statistically analyze and summarize large-scale fluorescence auto- and cross-correlation spectroscopy (FCS/FCCS) data. Fluctuation Statistics can read results files from the accompanying Fluctuation Analyzer software, applies required calibrations, normalizations and corrections and creates HTML-formatted report sheets. It also permits users to interactively explore the biophysical parameters extracted from larger numbers of cellular FCS/FCCS measurements.

Provides a standardized environment for the analyses of correlation data. PyCorrFit is delivered with several integrated model functions, covering a wide range of applications in standard confocal fluorescence correlation spectroscopy (FCS). It also contains equations dealing with different stimulation geometries. This method was developed to support many data file formats and to feature a set of tools specialized in data evaluation.

Evaluates image stacks acquired in imaging fluorescence correlation spectroscopy (FCS). ImFCS is a data analysis tool for camera based FCS that calculates a variety of spatiotemporal auto- and cross-correlations and differences between spatial forward and backward cross-correlations. It can facilitate the analysis imaging FCS data for the community interested in the dynamic behavior of molecules in model and living systems.

Extracts fluctuating fluorescence from measurements. PyScanFCS serves especially for perpendicular line scanning fluorescence correlation spectroscopy.

Reads flow cytometry standard (FCS) files. FCSParser allows users to exploit data from flow cytometry experiments.

Provides a visual basic for application (VBA) macro to be used with Zeiss confocal microscopes. MyPiC allows autofocus based on reflection and fluorescence multi-location time series and fluorescence-based tracking using the center of mass of the fluorescence signal. It also permits flexible combination of several independent Z-stack and channel settings and of imaging with fluorescence correlation spectroscopy (FCS) experiments.

Represents an integrated solution for data analysis and acquisition. SymPhoTime 64 permits users to target principally the results rather than the data processing. The interface of this tool simplifies the utilization by the user for an individual analysis or measurement process. Different data acquisition modes including interleaved trains of excitation and stimulated emission depletion (STED) laser pulses are integrated in a graphical user interface (GUI).

Permits users to process images and fluorescence correlation spectroscopy (FCS) data for FCS-calibrated imaging and high-throughput FCS. FCSAnalyze works in several steps and (i) generates bleach corrected correlation functions, (ii) browses through images and FCS positions, (iii) fits FCS data, (iv) computes the parameters of the calibration curve, (v) converts fluorescence intensities into concentrations and number of proteins.

Assists users with data evaluation. QuickFit is an open-source software for flow cytometry standard (FCS) and imagingFCS (imFCS) measurement. It also provides microscope characterization plugins such as selective plane illumination microscopy (SPIM) lightsheet characterization, camera calibration or colocalization analysis. It also includes a customizable user interface and a spectra viewer with comprehensive set of spectra.

Performs imaging and fluorescence cross-correlation spectroscopy (FCCS) measurements. FcsRunner can be used to create data for FCS-calibrated imaging. FCS measurements are performed at multiple stage positions, typically one stage position per cell. At each stage position, the user only specifies FCS points. After all positions have been specified the users starts the acquisition. Then for each position, an image is acquired, and FCS measurements are performed. The image and FCS settings are finally given.

Decompresses files with Zeiss fluorescence correlation spectroscopy (FCS) data and can display the two channels. FCSViewer exploits time calipers to select and enlarge any time interval and this feature can be repeated until the full resolution of the data is available. This software is only compatible with raw data.

Uses for fluorescence image analysis, visualization, simulation, and acquisition. SimFCS is a scientific software for data form spectroscopy and fluorescence microscopy. It can be applied to Fluorescence Correlation Spectroscopy, Fluorescence Lifetime Imaging, Pair correlation function, Image Mean Square Displacement, Single Particle and Modulation Tracking, Raster and Spatio-temporal Image Correlation Spectroscopy and many other.