FCS Express protocols

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FCS Express specifications

Information


Unique identifier OMICS_27062
Name FCS Express
Software type Application/Script
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Mac OS, Windows
Computer skills Medium
Version 6.04.0034
Stability Stable
Free trial Yes
Registration required Yes
Maintained Yes

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  • person_outline FCS Express Team <>

FCS Express in pipeline

2017
PMCID: 5438137
PMID: 28542402
DOI: 10.1371/journal.pone.0177921

[…] using custom routines created using cellprofiler (broad institute, cambridge, ma, usa). the segmented images from cellprofiler were reconstructed into image montages of the cardiac lobe using fcs express 5 software (de novo software, los angeles, ca, usa). using fcs express 5 (de novo), the high expressing sma cells on the dot plot were gated and the corresponding cells in the image […]


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FCS Express in publications

 (942)
PMCID: 5943604
PMID: 29743632
DOI: 10.1038/s41598-018-25667-3

[…] depending on the egfp/dsred tagged constructs used for transfection. proliferation was analyzed by flow cytometry (facsaria ii, bd biosciences, usa) and the results were interpreted using software fcs express 6., all experiments were performed on at least three independent biological samples with two technical replicates. single factor anova or student’s t-test were used to make relevant […]

PMCID: 5940207
PMID: 29738575
DOI: 10.1371/journal.pone.0196994

[…] instructions (bd bioscience, usa). the readings were performed using the facscalibur (becton-dickinson, franklin lakes, nj, usa) flow cytometer located at the institute of biology of unicamp, using fcs express 5 plus, research edition software., three doses of 3x105 tdcs isolated from ova tolerant balb/c mice or ndcs isolated from naïve balb/c mice were injected intravenously into naive […]

PMCID: 5945982
PMID: 29780375
DOI: 10.3389/fmicb.2018.00867

[…] performed using a bd-facscalibur® or facsverse® flow cytometer (bd biosciences, san jose, ca, united states). analysis of the dot-plot or mean fluorescence intensity (mfi) was performed using the fcs express program (de novo software, glendale, ca, united states)., statistical analysis was performed using graphpad instat® version 6 (graphpad software, san diego, ca, united states). normality […]

PMCID: 5949935
PMID: 29774060
DOI: 10.1177/1758835918770984

[…] stages or cell death were analyzed by flow cytometry in the bd facs canto a equipment (bd biosciences, nj, usa). in every sample, 10,000 events were measured and data were analyzed with the de novo fcs express v6.03.0011 software., ea.hy926 cells were used for this essay. cells were serum-deprived for 24 h, and trypsinized in order to culture 10,000 cells in 500 μl of phenol red-free […]

PMCID: 5943503
PMID: 29774024
DOI: 10.3389/fimmu.2018.00872

[…] (biolegend) was used for detection. intracellular staining was performed using the cytofix-cytoperm kit (bd). measurements were performed in a canto ii flow cytometer (bd) and analyzed using fcs express 6.0 (de novo software)., cells (107) were harvested, washed twice in cold pbs, and snap-frozen in liquid n2. pellets were resuspended in 500 µl of lysis buffer (buffer a: tris–hcl 50 mm […]


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FCS Express review

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Sean Burke

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Desktop
Great software!