Computational protocol: A novel alphaproteobacterial ectosymbiont promotes the growth of the hydrocarbon-rich green alga Botryococcus braunii

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Protocol publication

[…] 16S rDNA of BOTRYCO-2 was PCR amplified using the bacterial universal primer pair 27F/1492R. The determined 16S rDNA sequence of the bacterium has been deposited in the DNA Databank of Japan (DDBJ) under the accession number AB900796. Phylogenetic analyses were performed on the basis of neighbour-joining (NJ) and maximum-likelihood (ML) methods using MEGA version 5.2 and RAxML, respectively. The detailed protocols for DNA extraction, PCR, and sequencing reactions are described in . […]

Pipeline specifications

Software tools MEGA, RAxML
Databases DDBJ
Application Phylogenetics
Organisms Botryococcus braunii, Microcystis aeruginosa
Chemicals Hydrocarbons