|Number of samples:||3|
|Release date:||Jan 19 2015|
|Last update date:||Jan 20 2015|
|Dataset link||Drosophila miR-277 controls branched-chain amino acid catabolism and affects lifespan|
Transgenic Drosophila melanogaster fruitflies carrying strong, ubiquitously expressed pre-miR277 hairpins (wt and two mutant versions) were dissected, total RNA was extracted from the abdomen and gel-purified for size selection (~18-30 nt). Digested plasmid samples were compared to those of circular plasmids and a nontransfected control. The purpose of this experiment was to demonstrate the extent of expression from mutant pre-miR277 hairpins, mut1 should abolish Drosha-processing while mut2 is conservative.
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