Dataset features

Specifications


Application: sRNA-seq analysis
Number of samples: 3
Release date: Jan 19 2015
Last update date: Jan 20 2015
Access: Public
Chemicals: Amino Acids
Dataset link Drosophila miR-277 controls branched-chain amino acid catabolism and affects lifespan

Experimental Protocol


Transgenic Drosophila melanogaster fruitflies carrying strong, ubiquitously expressed pre-miR277 hairpins (wt and two mutant versions) were dissected, total RNA was extracted from the abdomen and gel-purified for size selection (~18-30 nt). Digested plasmid samples were compared to those of circular plasmids and a nontransfected control. The purpose of this experiment was to demonstrate the extent of expression from mutant pre-miR277 hairpins, mut1 should abolish Drosha-processing while mut2 is conservative.

Repositories


GEO

GSE42442

ENA

SRP017271

BioProject

PRJNA181983

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Contact


Klaus Förstemann

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