Computational protocol: Multilocus genotypes and broad host-range of Enterocytozoon bieneusi in captive wildlife at zoological gardens in China

Similar protocols

Protocol publication

[…] All nucleotide sequences obtained in this study were aligned with E. bieneusi reference sequences downloaded from the GenBank database using Blast [] and ClustalX software []. Phylogenetic analysis of ITS sequences was performed using Mega software [], and Maximum Likelihood analysis of the aligned E. bieneusi sequences was utilized to support genotype classifications. A total of 1,000 replicates were used for bootstrap analysis. […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Enterocytozoon bieneusi, Homo sapiens, Felis catus, Antilope cervicapra, Axis porcinus, Helarctos malayanus, Ursus arctos
Diseases Zoonoses, Microsporidiosis