Computational protocol: Excavation of Pid3 Orthologs with Differential Resistance Spectra to Magnaporthe oryzae in Rice Resource

Similar protocols

Protocol publication

[…] Sequences were aligned using DNAMAN (http://www.lynnon.com/), Clustal X version 2.0 , DNAStar (https://www.dnastar.com/products/lasergene.php) and manually edited using BioEdit version 7.0.1 . Protein motif search was performed using the SMART program (http://smart.embl-heidelberg.de/) and the RCM program (http://144.92.198.58/main/main.php). MEGA 5 was used to build phylogenetic tree of Pid3 orthologs. Sliding-window analysis, polymorphism and neutral tests of different regions of the Pid3 orthologs were conducted using DnaSP version 4.0 . […]

Pipeline specifications

Software tools DNAMAN, Clustal W, BioEdit, MEGA, DnaSP
Application Phylogenetics
Organisms Oryza sativa, Magnaporthe oryzae
Diseases Nijmegen Breakage Syndrome