Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Dec 3 2009
Last update date: Aug 23 2018
Access: Public
Dataset link colonic vs bone marrow MSC

Experimental Protocol


Mesenchymal stem cells were isolated by fast plastic adherence after collagenase type I-mediated dissociation of the tissue of origin. Cells were maintained in culture in low glucose DMEM with 10mM HEPES and 10% FBS. Cells were passaged at 90-100% confluency. Approximately 4 million cells from four MSC lines, two from colon and two from bone marrow, were lysed between passages 5 and 10. Total RNA was isolated, amplified, and hybridized to Affymetrix MOE230.2 chips.

Repositories


GEO

GSE19233

ArrayExpress

E-GEOD-19233

BioProject

PRJNA120757

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Contact


Thaddeus Stappenbeck
Thaddeus S. Stappenbeck