Computational protocol: A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish

Similar protocols

Protocol publication

[…] We used RESCUE-ESE (http://genes.mit.edu/burgelab/rescue-ese/) () and ESE Finder 3.0 (http://rulai.cshl.edu/cgi-bin/tools/ESE3/esefinder.cgi?process=home) (; ) for predictions of exonic splicing enhancer in pycr1a exon 3 sequence. For the ESE Finder run, we chose to focus on the best hits for each of the SRSF splicing factors to simplify the output. The sequences of the identified ESEs were copied from the output HTML pages and used for . […]

Pipeline specifications

Software tools RESCUE-ESE, ESEfinder
Application WGS analysis
Organisms Danio rerio
Chemicals Nucleotides