Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 33
Release date: Nov 7 2008
Last update date: Aug 28 2014
Access: Public
Dataset link Two Supporting Factors Greatly Improve the Efficiency of Human iPS Cell Generation

Experimental Protocol


Total RNA from hFSF, hAFF, hES cells (H1, H7) and 7 established iPS cell lines were labeled with Cy5, hybridized to a human Oligo Microarray (Phalanx Human Whole Genome OneArrayâ„¢, Phalanx Biotech) according to the manufacturer's protocol. Three technical repetitions were performed. After hybridization, arrays were scanned using GenePix 4000B scanner (Molecular Devices) and processed using the GenePix Pro 6.0 software (Molecular Devices). After removing control probes, a 14/33 presence call (SNR>=5 and foreground-background>0) was used to filter probes for the 33 microarrays, resulting in 12311 probes for further quantile normalization.

Repositories


GEO

GSE12922

ArrayExpress

E-GEOD-12922

BioProject

PRJNA110837

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Contact


Hongkui Deng