Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Dec 25 2008
Last update date: Jul 1 2016
Access: Public
Diseases: Breast Neoplasms, Lung Neoplasms, Neoplasms, Prehypertension
Dataset link Identification of transcriptional changes induced by FHIT in lung cancer cells NCI-H460

Experimental Protocol


RNA was extracted from NCI-H460 lung cancer cells (FHIT-negative) transduced with AdFHIT or AdLACZ (control), 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step. The whole procedure was repeated twice (starting from thawing of new aliquot of NCI-H460 cells) resulting in two independent experiments (exp1 and exp2).

Repositories


GEO

GSE11559

BioProject

PRJNA106293

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Contact


Luca Roz