|Application:||Gene expression microarray analysis|
|Number of samples:||16|
|Release date:||Dec 25 2008|
|Last update date:||Jul 1 2016|
|Diseases:||Breast Neoplasms, Lung Neoplasms, Neoplasms, Prehypertension|
|Dataset link||Identification of transcriptional changes induced by FHIT in lung cancer cells NCI-H460|
RNA was extracted from NCI-H460 lung cancer cells (FHIT-negative) transduced with AdFHIT or AdLACZ (control), 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step. The whole procedure was repeated twice (starting from thawing of new aliquot of NCI-H460 cells) resulting in two independent experiments (exp1 and exp2).