Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 10
Release date: Nov 13 2009
Last update date: Jul 1 2016
Access: Public
Chemicals: Iron, Zinc, Lactic Acid
Dataset link Resistance to hop iso-α-acids in yeast involves active export and vacuolar sequestration

Experimental Protocol


Two complementary genome-wide approaches were employed to investigate cellular responses of S. cerevisiae to hop extracts enriched in iso-α-acids. Microarray transcriptome analysis was performed on chemostat cultures of an S. cerevisiae reference strain grown in the presence and absence of iso-α-acids. In addition, screening of the nearly complete set of yeast open reading frame (ORF) haploid knock-outs generated by the Saccharomyces Genome Deletion Project (SGDP) (Open Biosystems) identified the mutants with increased hop sensitivity. Subsequently, involvement of selected genes and cellular processes in hop acid sensitivity and tolerance was analyzed by construction and detailed analysis of selected mutant strains.

Repositories


GEO

GSE15094

ArrayExpress

E-GEOD-15094

BioProject

PRJNA114801

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Contact


Jean-Marc Daran