Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Dec 7 2009
Last update date: Oct 29 2018
Access: Public
Genes: CD36, CD9, TREM1, Tastin
Taxon: Homo sapiens, Mus musculus
Dataset link Comparison of gene expression profiles between human and mouse monocyte subsets [human data]

Experimental Protocol


The two major subsets of monocytes (Ly-6C+ and Ly-6Clo) from 12-week old C57Bl/6 mice were sorted and the RNA extracted and hybridized to Affymetrix GeneChip® 430 2.0 arrays. We pooled leukocytes from 5 mice for each sort and sorted 4 separate times for 4 biological replicates. The two major monocyte subsets (CD16- and CD16+) were isolated from venous heparinized blood from apparently healthy human volunteers using MACS technology with all reagents and tools from Miltenyi Biotec. Three separate donors were hybridized three different times to Affymetrix U133 Plus 2.0 array.

Repositories


GEO

GSE18565

ArrayExpress

E-GEOD-18565

BioProject

PRJNA121385

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Contact


Loems Ziegler-Heitbrock