|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Dec 7 2009|
|Last update date:||Oct 29 2018|
|Genes:||CD36, CD9, TREM1, Tastin|
|Taxon:||Homo sapiens, Mus musculus|
|Dataset link||Comparison of gene expression profiles between human and mouse monocyte subsets [human data]|
The two major subsets of monocytes (Ly-6C+ and Ly-6Clo) from 12-week old C57Bl/6 mice were sorted and the RNA extracted and hybridized to Affymetrix GeneChip® 430 2.0 arrays. We pooled leukocytes from 5 mice for each sort and sorted 4 separate times for 4 biological replicates. The two major monocyte subsets (CD16- and CD16+) were isolated from venous heparinized blood from apparently healthy human volunteers using MACS technology with all reagents and tools from Miltenyi Biotec. Three separate donors were hybridized three different times to Affymetrix U133 Plus 2.0 array.