Dataset features


Application: Gene expression microarray analysis
Number of samples: 5
Release date: Mar 13 2015
Last update date: May 15 2017
Access: Public
Genes: POU5F1, SOX2, KLF4
Taxon: Homo sapiens, Mus musculus
Dataset link A New Type of Human iPS Cells: Human Induced Hepatic Lineage-Oriented Stem Cells

Experimental Protocol

Using the AllPrep DNA/RNA Mini kit (Qiagen), total RNA was prepared from a hepatocellular carcinoma cell line (HuH-7) and human primary adult hepatocytes (obtained from the Health Science Research Resources Bank) as well as clones AFB1-1, NGC1-1, and NGC1-2 that were cultured on the MEFs (5 X 10^4 cells/cm^2) with mTeSR1 (StemCell Technologies) medium in gelatin-coated 100-mm dishes before long-term serial passage. The microarray study was carried out using a Whole Human Genome Oligo microarray 4X44K (Agilent). The analysis was performed by Bio Matrix Research according to Agilent technical protocols. Data from these experiments and the GEO database for hESCs (hES_ES01, GSM194392; hES_BG03, GSM194391; hES_H9, GSM194390) and hiPSCs (201B7, GSM241846) established from human adult fibroblasts (fibroblast, GSM242095) were analyzed with GeneSpring GX 11.5 software (Agilent). The 50th percentile of fluorescent intensity distribution was normalized across arrays.








Tetsuya Ishikawa

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