Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Sep 14 2013
Last update date: Dec 27 2017
Access: Public
Chemicals: Potassium
Dataset link Gene expression analysis of rice seedling under potassium deprivation

Experimental Protocol


Potassium treated seedlings were used for RNA extraction and hybridization on Affymetrix rice Genechip by microarrays. Three biological replicates of each sample were used for microarray analysis. We wanted to know the altered expression patterns of potassium-responsive genes majorly involved in metabolic processes, stress responses, signaling pathways, transcriptional regulation, and transport of multiple molecules including K+. Seeds of indica rice were surface sterilized and grown hydroponically in rice growing medium. Plants were grown in the rice growth room under the condition of 16 h light/8 h dark (28°C) photoperiod with 70 % humidity. After 5 days of normal growth, one half of the rice seedlings were transferred to nutrient media with normal concentration of potassium (KP, 0.409 mM K2SO4) and other one half to medium without potassium (KM, 0 mM). After 5 days, potassium (0.409 mM K2SO4) was resupplied to the plants grown in KM medium for 6 h.

Repositories


GEO

GSE44250

ArrayExpress

E-GEOD-44250

BioProject

PRJNA189246

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Contact


GIRDHAR PANDEY
GIRDHAR KUMAR PANDEY

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