Computational protocol: Glyceraldehyde-3-phosphate dehydrogenase gene over expression correlates with poor prognosis in non small cell lung cancer patients

Similar protocols

Protocol publication

[…] RNA was isolated from paraffin-embedded tumor samples using the High Pure FFPE RNA Micro Kit (Roche Applied Science, Mannheim, Germany) with minor modifications. RNA were reverse-transcribed with SuperScript™ II RT (Invitrogen, Grand Island, NY, USA) according to the manufacturer’s instructions. Resulting cDNA was amplified by the LightCycler 480 Real Time PCR System II (Roche Applied Science). Relative gene expression levels were calculated using the Qgene software [] featuring an efficiency corrected threshold cycle based algorithm. Beta-2-microglobulin (B2M) and beta-glucuronidase (GUSB) were used as housekeeping genes and a virtual housekeeping gene was calculated using BestKeeper software []. PCR primer sequences are reported in Additional file . […]

Pipeline specifications

Software tools QGene, BestKeeper
Application qPCR
Organisms Homo sapiens
Diseases Carcinoma, Non-Small-Cell Lung, Lung Neoplasms, Neoplasms
Chemicals Glucose, Glyceraldehyde 3-Phosphate, Oxygen