Dataset features

Specifications


Application: RNA-seq analysis, sRNA-seq analysis
Number of samples: 2
Release date: Oct 1 2008
Last update date: Feb 18 2014
Access: Public
Chemicals: Nucleotides
Dataset link Small RNA SBS signatures identified from maize using Illumina's SBS technology

Experimental Protocol


This library was derived from maize variety K55 wild-type and mop1 mutant. Immature ears were harvested from plants grown outdoors in Tuscon, AZ. The material was harvested from plants 68 days after germination, with the floral tissue including young tassels 2-3 cm long. Total RNA was isolated using a standard TRIZOL-based method and the small RNA libraries were generated as described by Lu et al. (Methods 2007, 43:110), followed by sequencing with Illumina's SBS method. The adapter sequences were removed, and the abundance of each distinct small RNA was determined. Raw data are unavailable from Illumina

Repositories


GEO

GSE12173

ArrayExpress

E-GEOD-12173

BioProject

PRJNA113465

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Contact


Blake Meyers
Blake C. Meyers