Computational protocol: Nerve degeneration and regeneration in the cephalopod mollusc Octopus vulgaris: the case of the pallial nerve

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Protocol publication

[…] Images were processed with IMARIS 64 7.5 (Bitplane Inc.) for nuclear and mitotic cells counting and neurofilament area calculation. The analysis has been carried out from ten serial sections (stacks, 20 μm thick) of each area of interest (N = 9, three for each time-point). Statistics and box-and-whisker plots were generated using SPSS version 14.0 (SPSS Inc). Data were tested for normality and Student’s t-test was applied according to Zar. Differences were considered significant at p < 0.05. […]

Pipeline specifications

Software tools Imaris, SPSS
Application Miscellaneous
Organisms Octopus vulgaris