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Protocols

FibrilTool specifications

Information


Unique identifier OMICS_10572
Name FibrilTool
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java
Computer skills Medium
Stability Stable
Requirements
ImageJ
Maintained Yes

Versioning


No version available

Publication for FibrilTool

FibrilTool citations

 (20)
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Connexin 30 controls astroglial polarization during postnatal brain development

2018
PMCID: 5869003
PMID: 29475972
DOI: 10.1242/dev.155275
call_split See protocol

[…] ed 8 h after wounding. Wounded astrocyte cultures were fixed with PFA and immunostained for tubulin. Confocal z-stack images of individual wound-edge astrocytes were analyzed using the ImageJ plug-in FibrilTool (). Briefly, regions of interest were drawn around a single-cell tubulin cytoskeleton and the average fibril orientation provided by the plug-in was compared with the actual angle of the ax […]

library_books

Aβ mediates F actin disassembly in dendritic spines leading to cognitive deficits in Alzheimer's disease

2018
PMCID: 5792472
PMID: 29246925
DOI: 10.1523/JNEUROSCI.2127-17.2017

[…] geJ plugin called Ridge Detection (). In addition to the detection of these rods, we were able to automatically quantify the orientation of the radiating structures using another ImageJ plugin called FibrilTool (). FibrilTool calculates a value referred to as “anisotropy,” which is a measure of how parallel the fibers are with respect to each other. Mean anisotropy values were compared between spi […]

library_books

A tension adhesion feedback loop in plant epidermis

2018
eLife
PMCID: 5963923
PMID: 29683428
DOI: 10.7554/eLife.34460.027

[…] rmal cortical microtubules signal. Further analysis was performed in 2D using the imageJ morpholibJ library (), to segment cells, the analyze particle tool to define ROIs and an automated version the FibrilTool macro () to analyze the principal orientation (θM) and anisotropy of the CMT arrays (see ).For the CMT response to ablation, only the cells directly surrounding the ablation were analyzed. […]

library_books

PAWS1 controls cytoskeletal dynamics and cell migration through association with the SH3 adaptor CD2AP

2018
PMCID: 5818054
PMID: 29175910
DOI: 10.1242/jcs.202390

[…] by widefield deconvolution microscopy. To assess cell actin organization in phalloidin-stained WT, PAWS1−/− and CD2AP−/− U2OS cells, the actin area was bounded in ImageJ then submitted to the plugin Fibriltool to determine anisotropy in response to genotype (). Overlapping cells or those undergoing division were omitted from analysis. […]

library_books

Dynamic regulation of TREK1 gating by Polycystin 2 via a Filamin A mediated cytoskeletal Mechanism

2017
Sci Rep
PMCID: 5727099
PMID: 29234037
DOI: 10.1038/s41598-017-16540-w

[…] wares (Applied Precision/GE Deltavision) were used for image acquisition and structured illumination reconstruction, respectively. Analysis of SIM data was performed using ImageJ (NIH) and the plugin FibrilTool to calculate anisotropy, the property of being directionally dependent, amongst actin filaments. The cell anisotropy values were measured by analyzing 4μm thickness of actin staining just u […]

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Cyclic Tensile Strain Can Play a Role in Directing both Intramembranous and Endochondral Ossification of Mesenchymal Stem Cells

2017
Front Bioeng Biotechnol
PMCID: 5712005
PMID: 29230389
DOI: 10.3389/fbioe.2017.00073
call_split See protocol

[…] ices were washed in PBS and imaged with an Olympus FluoView FV1000 Confocal Microscope. Anisotropy of actin filaments was quantified by performing analysis of confocal images (n = 4) using the ImageJ FibrilTool plugin (Boudaoud et al., ). […]


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FibrilTool institution(s)
Laboratoire de Reproduction et Développement des Plantes, Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS), Ecole Normale Supérieure de Lyon (ENS), Université de Lyon, Lyon, France

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