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File manipulation software tools | Sanger sequencing data analysis

Although there has been a massive development of next generation sequencing recently, Sanger sequencing method still remains widely used for routine sequencing of DNA fragments (Chen et al., 2014; Dawit et al., 2014; Ihle et al., 2014; Rounds et al., 2014). Results produced by capillary sequencing machines are often analyzed by Data Collection software, which generates ABIF (Applied Biosystems Genetic Analysis) formatted files (.ab1, .fsa).

(Chen et al., 2014) Rapid Sanger sequencing of the 16S rRNA gene for identification of some common pathogens. PLoS One.
(Dawit et al., 2014) Sanger sequencing exons of BDNF gene derived from a Latino sample. Neurology.
(Ihle et al., 2014) Comparison of high resolution melting analysis, pyrosequencing, next generation sequencing and immunohistochemistry to conventional Sanger sequencing for the detection of p. V600E and non-p. V600E BRAF mutations. BMC Cancer.
(Rounds et al., 2014) Predicting conversion to multiple sclerosis using antibody genetics: comparing Sanger and next generation sequencing methods. J. Immunology.

Source text:
(Beran et al., 2015) ABIF Manager: simple reading and editing of ABIF formatted files. Bioinformatics.

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