Automates design of multiple-point thermostable mutant proteins which combines structural and evolutionary information in its calculation core. FireProt uses sixteen tools and three protein engineering strategies for making reliable protein designs. It allows user to analyze and modify the design of thermostable mutants. The graphical user interface (GUI) provides user to interactively analyze individual mutations selected as a part of energy, or evolution-based approach together with the ability to design their own multiple-point.
Loschmidt Laboratories, Department of Experimental Biology, Masaryk University, Brno, Czech Republic; Department of Information Systems, Faculty of Information Technology, Brno University of Technology, Brno, Czech Republic; International Centre for Clinical Research, St. Anne’s University Hospital Brno, Brno, Czech Republic; Centre of Excellence IT4Innovations, Technical University Ostrava, Ostrava
FireProt funding source(s)
This work was supported by Ministry of Education, Youth and Sports of the Czech Republic from the National Programme of Sustainability II [LQ1605, LO1214], European Regional Development Fund [ELIXIR-CZ LM2015047], Grant Academy of the Czech Republic [16-06096S] and by Brno University Technology [FIT-S- 17-3964].