FitCTF specifications


Unique identifier OMICS_27277
Name FitCTF
Alternative name FitCTF2
Software type Application/Script
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages MATLAB, Python
Computer skills Advanced
Stability Stable
Registration required Yes
Maintained Yes


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  • person_outline Wen Jiang <>
  • person_outline Chao Yang <>

Additional information

Publications for FitCTF

FitCTF in publications

PMCID: 4988150
PMID: 27279624
DOI: 10.1128/JVI.00746-16

[…] defocus of each micrograph was determined using the program from the eman2 suite (). particle images were phase corrected for the effect of the contrast transfer function using the program (). particles were separated into two halves (odd and even image numbers) and treated completely independently in according to the gold standard approach (). first, nine sets of 400 […]

PMCID: 4777393
PMID: 26938634
DOI: 10.1371/journal.ppat.1005454

[…] using motioncorr []. due to the sticky nature of the sample, particles were manually boxed using the program from the eman2.1 package []. ctf fitting was automatically performed using the program in jspr package [], then visually validated and adjusted using eman1.9 ctfit program []. the structure factor was determined by computational fitting of multiple micrographs […]

PMCID: 4346626
PMID: 25698059
DOI: 10.1038/ncomms7341

[…]  °c) and 4,035 (denv3 at 37 °c) particles were used for reconstruction. the parameters of the contrast transfer function for each micrograph were first automatically determined by using the programme fitctf and then manually optimized using the ctfit programme in eman., for the denv3 at 28 °c control, the cryo-em map of denv4 (electron microscopy database accession code emd-2485) was used as an in […]

PMCID: 2834796
PMID: 20090755
DOI: 10.1038/nature08701

[…] () in contrast to an earlier report showing multiple conformations in the apo state. contrast transfer functions (ctf) of these particles were first fitted using automatic ctf fitting program and then manually examined and adjusted using the eman program ctfit., all these particles were phase-flipped, and then an initial model of each of the four states was generated using […]

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FitCTF institution(s)
Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, IN, USA
FitCTF funding source(s)
Supported by grants from NIH (S10RR023011, P01AI055672, R01AI072035).

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