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flexImaging specifications


Unique identifier OMICS_05684
Name flexImaging
Software type Toolkit/Suite
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Unix/Linux
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes


No version available

flexImaging citations


Membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic ischaemic insult

Sci Rep
PMCID: 5934395
PMID: 29725040
DOI: 10.1038/s41598-018-25088-2

[…] and metabolites were ionized/desorbed using a 100-µm raster step size with a SmartBeam II laser (2 kHz, 200 shots/pixel) and 16,000 resolution (FWHM) at m/z 400. The raw MSI data were analysed using FlexImaging 4.1 (Bruker Daltonics, Bremen, Germany), transformed into an imzML file and processed using CycloBranch. […]


Spatial Systems Lipidomics Reveals Nonalcoholic Fatty Liver Disease Heterogeneity

Anal Chem
PMCID: 5906754
PMID: 29570976
DOI: 10.1021/acs.analchem.7b05215

[…] The raw MALDI-TOF data was loaded into Fleximaging software v.4.1 (Bruker Daltonik GmbH, Bremen, Germany), where the MSI image was semiautomatically overlaid (using fiducial markers) with a histological optical image of the same tissue sec […]


Stearate‐to‐palmitate ratio modulates endoplasmic reticulum stress and cell apoptosis in non‐B non‐C hepatoma cells

Cancer Sci
PMCID: 5891190
PMID: 29427339
DOI: 10.1111/cas.13529
call_split See protocol

[…] ere set to obtain the highest sensitivity with m/z values in the range of 200‐1000 in the negative‐ion mode. The automatic acquisition of spectra and reconstruction of ion images were performed using FlexImaging Software (Bruker Daltonics). […]


Lactate Induced Glucose Output Is Unchanged by Metformin at a Therapeutic Concentration – A Mass Spectrometry Imaging Study of the Perfused Rat Liver

Front Pharmacol
PMCID: 5827415
PMID: 29520235
DOI: 10.3389/fphar.2018.00141
call_split See protocol

[…] e, acquiring in the 20–500 Da mass range and summing 2000 laser shots per raster position. For analyses of the fragmentation of the parent ion measurements were made in LIFT MS/MS mode. The resulting FlexImaging 4.1 (Bruker Daltonics, Bremen, Germany) files, containing the acquired spectra from the measurement regions, were imported into SCiLS Lab 2017a software (Bremen, Germany) for subsequent To […]


Layer specific lipid signatures in the human subventricular zone demonstrated by imaging mass spectrometry

Sci Rep
PMCID: 5803191
PMID: 29416059
DOI: 10.1038/s41598-018-20793-4
call_split See protocol

[…] solarix-XR mass spectrometer (Bruker Daltonics, Bremen, Germany) with MALDI ionisation and at 20 µm spatial resolution. Spectra were collected in the m/z range of 150–2000. FT-ICR data were read into flexImaging v4.1 (Bruker Daltonics, Bremen, Germany) and normalised using the root mean square method. Peak centroids were taken as accurate masses to facilitate the assignment of lipid identities. […]


MALDI Mass Spectral Imaging of Bile Acids Observed as Deprotonated Molecules and Proton Bound Dimers from Mouse Liver Sections

J Am Soc Mass Spectrom
PMCID: 5889423
PMID: 29417494
DOI: 10.1007/s13361-017-1886-6

[…] on window: 3–5 u) and accumulation in the hexapole for collision-induced dissociation (CID) at varying collision energies (5–55 V). Data were processed and analyzed using the Bruker Data Analysis and FlexImaging software for single mass spectra and imaging data sets, respectively. Mass spectral interpretations were conducted using the METLIN and LIPID MAPS databases to match the accurate masses of […]


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flexImaging institution(s)
Bruker, MA, USA

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