bookmark FLIMfit Allows the analysis of fluorescence lifetime imaging (FLIM) data based on variable projection. FLIMFit enables to apply global analysis to obtain quantitative information from large multi-well plate or time-series datasets. It is able to globally fit complex decay models to photon-constrained data. With this tool, data can be fitted more robustly to more complex decay models. It applies global fitting to analyse polarisation resolved time-correlated single photon counting (TCSPC) data to determine the anisotropy decay and lifetime parameters associated with homo-Forster Resonant Energy Transfer (FRET).