Fluorescence-lifetime microscopy data analysis software tools | Laser scanning microscopy
Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. This technique can provide information, not only concerning the localization of specific fluorophores, but also about the local fluorophore environment. It can be used in scanning confocal, multi-photon microscopes, or in wide-field microscopes and endoscopes.
Allows users to analyze images obtained through Fluorescence Lifetime Imaging Microscopy (FLIM) experiments. TIMP is a package that provides functions to assist in visual interpretation and validation of the results of model fit as well as options for numerical convolution with a measured instrument response function (IRF). The application proposes a partitioned variable projection algorithm with the aim of increasing the rapidity of the processing.
Allows the analysis of fluorescence lifetime imaging (FLIM) data based on variable projection. FLIMFit enables to apply global analysis to obtain quantitative information from large multi-well plate or time-series datasets. It is able to globally fit complex decay models to photon-constrained data. With this tool, data can be fitted more robustly to more complex decay models. It applies global fitting to analyse polarisation resolved time-correlated single photon counting (TCSPC) data to determine the anisotropy decay and lifetime parameters associated with homo-Forster Resonant Energy Transfer (FRET).
Allows the analysis of fluorescence lifetime imaging ophthalmoscopy (FLIO) data. FLIMX corrects the influence of the crystalline lens fluorescence on the approximated fluorescence lifetime of the retina. It implements known multi-exponential and stretched exponential approaches, as well as new layer-based multi-exponential approaches. It also grafts stochastic and deterministic minimization algorithms to determine the fluorescence lifetime parameters.
Automates processing of intensity-based cell image segmentation. FLIM-FRET analyzer can separate objects of interest from the background to delineate whole cells. It simplifies image segmentation into single cells followed by donor lifetime and donor/acceptor fluorescence intensity quantification. For the software to work, users have to create a FRET collection which associates fluorescent intensity and fluorescence decay image datasets. To create a FRET collection, users import the donor and acceptor fluorescent channels and the donor fluorescence decay curves.
Represents an integrated solution for data analysis and acquisition. SymPhoTime 64 permits users to target principally the results rather than the data processing. The interface of this tool simplifies the utilization by the user for an individual analysis or measurement process. Different data acquisition modes including interleaved trains of excitation and stimulated emission depletion (STED) laser pulses are integrated in a graphical user interface (GUI).
Offers a library for exponential curve fitting. SLIM Curve is an open source module that can be used through two different software: (i) TRI2 to analyze fluorescence lifetime microscopy (FLIM) data; and (ii) ImageJ. As a plugin of ImageJ, the software permits the analysis of FLIM and Spectral Lifetime Imaging (SLIM) data and contains features allowing users to perform stretched exponential fits, triple exponential fits and a function for exporting results to text format for further analysis.
Assists users to perform fluorescence decay analysis. DecayFit is a program that includes several features allowing researchers to execute several works: analyze time-resolved fluorescence decays by iterative reconvolution or modified tail fitting; global optimization: analysis of multiple decays simultaneously; time-resolved forster resonance energy transfer (FRET) analysis; or parameter confidence interval estimation.
Manages instrument control, image processing and data analysis. SlideBook can drive hundreds of devices including microscopes, stages, lasers, wheels, piezos, scanners or shutters. It acquires data in 3D format over time, color, and specimen locations in customizable experiment protocols. This tool offers a solution to investigate images and obtain statistical data via a wide variety of algorithms while maintaining original data integrity.
Uses for fluorescence image analysis, visualization, simulation, and acquisition. SimFCS is a scientific software for data form spectroscopy and fluorescence microscopy. It can be applied to Fluorescence Correlation Spectroscopy, Fluorescence Lifetime Imaging, Pair correlation function, Image Mean Square Displacement, Single Particle and Modulation Tracking, Raster and Spatio-temporal Image Correlation Spectroscopy and many other.
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