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Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. This technique can provide information, not only concerning the localization of specific fluorophores, but also about the local fluorophore environment. It can be used in scanning confocal, multi-photon microscopes, or in wide-field microscopes and endoscopes.
(Korczynski., 2009) [Fluorescence lifetime imaging microscopy (FLIM) in biological and medical research]. Postepy Biochem.