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FLOCK specifications


Unique identifier OMICS_07036
Alternative name FLOw Clustering without K
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages C
Computer skills Advanced
Stability Stable
Maintained Yes




No version available


  • person_outline Richard H. Scheuermann

Publication for FLOw Clustering without K

FLOCK citations


ImmPort, toward repurposing of open access immunological assay data for translational and clinical research

Sci Data
PMCID: 5827693
PMID: 29485622
DOI: 10.1038/sdata.2018.15

[…] e framework established by the Galaxy Project established in making code freely available to the wider scientific community. The adaptation of the Galaxy framework to host flow analysis tools such as FLOCK and FlowSOM also addresses several data analysis challenges identified by ImmPort users in providing easier file upload, support for high-throughput analysis, and flexibility to integrate new to […]


Automated Analysis of Flow Cytometry Data to Reduce Inter Lab Variation in the Detection of Major Histocompatibility Complex Multimer Binding T Cells

Front Immunol
PMCID: 5526901
PMID: 28798746
DOI: 10.3389/fimmu.2017.00858

[…] FCS files were uploaded to FLOCK at www.immport.niaid.nih.gov and joined in datasets for each individual lab. The files were then initially analyzed as a dataset using FLOCK version 1.0 with the parameters set at auto. Unused m […]


CD4+ T cells with an activated and exhausted phenotype distinguish immunodeficiency during aviremic HIV 2 infection

PMCID: 5051526
PMID: 27525551
DOI: 10.1097/QAD.0000000000001223

[…] ad than the HIV-1-infected individuals (see Supplemental Digital Content Table S1).Distribution of populations defined by (a) expression of activation/exhaustion markers (CD38, HLA-DR, and PD-1), (b) FLOCK analysis, and (c) PCA histogram comparing viremic and aviremic HIV-2-infected groups. SPICE analysis: median and IQR are shown for all scatter plots/columns and Mann–Whitney tests were performed […]


Immunophenotype Discovery, Hierarchical Organization, and Template Based Classification of Flow Cytometry Samples

PMCID: 5005935
PMID: 27630823
DOI: 10.3389/fonc.2016.00188

[…] arm, manual gating is not feasible for high-dimensional or high-throughput FC data. To address the gating problem, researchers have proposed several automated clustering algorithms, such as FLAME (), FLOCK (), flowClust (), flowMeans (), SamSPECTRAL (), SWIFT (), etc. Aghaeepour et al. () provides a state-of-the-art summary of the field.Different algorithms perform better for different FC datasets […]


Avian Influenza A(H5N1) Virus in Egypt

PMCID: 4766899
PMID: 26886164
DOI: 10.3201/eid2203.150593

[…] norate, the same locations are continuously sampled by a poultry veterinarian, who collects 1 swab sample per bird; depending on the size of the poultry population, as many as 5 birds are sampled per flock. Birds are not randomly selected, and samples also are collected from sick or dead birds found onsite. Samples are mostly from chickens, but ducks, geese, turkeys, pigeons, and quails also are s […]


Increased NK Cell Maturation in Patients with Acute Myeloid Leukemia

Front Immunol
PMCID: 4635854
PMID: 26594214
DOI: 10.3389/fimmu.2015.00564

[…] ols are then required to address these problems. Recently, algorithms for automatic gating and 2D mapping of high dimensional dataset have been developed, such as Spade (), viSNE (), flowClust (), or FLOCK (, ). These algorithms combined to classification methods enable the visualization of multiple parameters and summarize information. These approaches are of particular importance to enable data […]


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FLOCK institution(s)
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA

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