FlowCal statistics

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FlowCal specifications


Unique identifier OMICS_11894
Name FlowCal
Software type Package/Module
Interface Command line interface, Graphical user interface
Restrictions to use None
Input data FCS files
Operating system Unix/Linux, Mac OS, Windows
Programming languages Python
Computer skills Advanced
Version 1.0.0
Stability Stable
Microsoft Excel
Maintained Yes


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  • person_outline Jeffrey J. Tabor <>

Publication for FlowCal

FlowCal in publications

PMCID: 5897336
PMID: 29650958
DOI: 10.1038/s41467-018-03929-y

[…] scatter (ssc) gate. rainbow calibration beads from spherotech, inc. (cat. no. rcp-30-20a) were also collected each day at identical detector gain settings. flow cytometry data were processed with flowcal. events were selected by discarding the first 250 and last 100 time ordered events, a density gate was then applied to select the densest 10% of events (~1000–2000 events) in fsc/ssc space […]

PMCID: 5408778
PMID: 28438832
DOI: 10.15252/msb.20167456

[…] bead sample, and at least 20,000 events were collected for each culture sample., single‐cell distributions of sfgfp fluorescence were gated, analyzed, and calibrated into mefl and mecy units using flowcal (castillo‐hair et al, ) via a custom python script (). measurements were gated on the fsc and ssc channels using a gate fraction of 0.3 for calibration beads and 0.8 for cellular samples […]

PMCID: 5408782
PMID: 28373240
DOI: 10.15252/msb.20167416

[…] calibration particles (spherotech, catalog rcp‐30‐20a) were run at the end of every experiment at the gain settings used for data collection., after data acquisition, raw data were processed using flowcal (castillo‐hair et al, ). first, a standard curve was created from the calibration beads to convert arbitrary units into absolute fluorescence units (mefl for fl1 and mecy for fl3). second, […]

PMCID: 5096413
PMID: 27805047
DOI: 10.1038/srep35363

[…] were measured at each session. data was processed and sfgfp and mcherry fluorescence were calibrated to molecules of equivalent fluorescein (mefl) and molecules of equivalent cy5 (mecy) with flowcal., data was modeled as previously. parameter fitting was performed in graphpad. all parameters were constrained to >0, and τdelay and kg are shared between step-on and step-off data sets., […]

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FlowCal institution(s)
Department of Bioengineering, Rice University, Houston, TX, USA; Center for Theoretical Biological Physics, Rice University, Houston, TX, USA; Department of Biosciences, Rice University, Houston, TX, USA
FlowCal funding source(s)
The National Science Foundation (EFRI-1137266 and MCB-1244135); the Office of Naval Research (MURI N000141310074, YIP N000141410487); the National Institutes of Health (1R21AI115014-01A1); the Welch Foundation (C-1856); an NSF Graduate Research Fellowship (DGE-0940902); an NDSEG Fellowship

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