FlowJo pipeline

FlowJo specifications

Information


Unique identifier OMICS_05624
Name FlowJo
Software type Package/Module
Interface Command line interface
Restrictions to use License purchase required
Operating system Unix/Linux, Mac OS, Windows
License Commercial
Computer skills Advanced
Version 10.2
Stability Stable
Maintained Yes
Wikipedia https://en.wikipedia.org/wiki/FlowJo

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FlowJo citations

 (3)
2018
PMCID: 5869589

[…] from a member of family b (b5) carrying ikzf1 mutation and a travel control were analysed using an 18-channel (16 fluorochromes, 2 light scatter) panel. compensated fcs files were manually gated (flowjo 10.1r5, treestar, inc.) to export lin−dr+cd14− cells., flowsom 1.7.1 was used for further analysis. from each of the two files, 550 cells were randomly selected. a total of 12 surface markers […]

2017
PMCID: 5607261

[…] negative and single-stained compensation beads (ebioscience) were acquired for each experiment, before sample acquisition, and used to calculate the compensation matrix. data was analysed using flowjo version 9.9.4 software (treestar) as well as pestle v1.8 and spice (simplified presentation of incredibly complex evaluations) v5.142., cell counts were analysed using the combinatorial […]

2017
PMCID: 5459756

[…] were run through flowai v1.2.4, a quality control package that filters events affected by technical variation, such as abrupt flow rate fluctuations (monaco et al., 2016). gating was performed in flowjo v.10.1 (flowjo, llc). subsequent analysis was performed using flowsom (v1.2.0), an r bioconductor package that uses self-organizing maps for dimensional reduction visualization of flow […]

FlowJo review

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Arup Ghosh

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It's an all in one suite for FACS data analysis.