FlowJo statistics

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Citations per year

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Protocols

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FlowJo specifications

Information


Unique identifier OMICS_05624
Name FlowJo
Software type Package/Module
Interface Command line interface
Restrictions to use License purchase required
Operating system Unix/Linux, Mac OS, Windows
License Commercial
Computer skills Advanced
Version 10.2
Stability Stable
Maintained Yes
Wikipedia https://en.wikipedia.org/wiki/FlowJo

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  • person_outline FlowJo Team <>

FlowJo in pipelines

 (2)
2017
PMCID: 5459756
PMID: 28343983
DOI: 10.1016/j.stem.2017.02.014

[…] fcs files were run through flowai v1.2.4, a quality control package that filters events affected by technical variation, such as abrupt flow rate fluctuations (). gating was performed in flowjo v.10.1 (flowjo, llc). subsequent analysis was performed using flowsom (v1.2.0), an r bioconductor package that uses self-organizing maps for dimensional reduction visualization of flow […]

2016
PMCID: 5179518
PMID: 28066414
DOI: 10.3389/fimmu.2016.00603

[…] and negative populations., flow cytometry data analysis was performed using the r programming language for statistical computing including the flowcore (), flowstats () r packages, or with the flowjo software (tree star). gating strategy involved the following sequential steps: gating on lymphocytes based on forward and side scatter, doublet-exclusion based on pulse width versus forward […]


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FlowJo in publications

 (20282)
PMCID: 5959860
PMID: 29777137
DOI: 10.1038/s41467-018-04315-4

[…] cells were washed three times in pbs with 1% bsa. ten thousand events were recorded and gated for cy5.5 signal on a facs canto ii flow cytometer. percent cy5.5 positive cells were quantified using flowjo® software. data are presented as mean ± sem from three biological replicates. statistical significance determined using student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001)., ncr […]

PMCID: 5959879
PMID: 29777108
DOI: 10.1038/s41467-018-04355-w

[…] cubated with antibody for 30 min on ice, in the dark. cells were then washed two times with facs buffer and taken for flow cytometry analysis on an lsrfortessa. the collected data were analyzed using flowjo software v10. when performing facs on lung tissues, siglec-f positive cells were included with dead cells to remove alveolar macrophages and/or eosinophils. ims were identified as cd45 + /cd11b […]

PMCID: 5959866
PMID: 29777171
DOI: 10.1038/s41467-018-04329-y

[…] 553356 = cell, clone 2b8), anti-mouse ly-6g (gr-1) (biolegend, 108411, clone rb6-8c5). samples were measured on lsr fortessa or canto ii flow cytometers (bd biosciences) and analyzed using flowjo software (tree star)., hek293 cells stably expressing pmscv-rtta3-ires-ecor-pgk-puro were transiently transfected with the indicated constructs using the pei transfection method. […]

PMCID: 5958132
PMID: 29773802
DOI: 10.1038/s41467-018-04418-y

[…] (#01-2222-42, affymetrix, inc.; san diego, ca). flow cytometry was performed using a bd fortessa flow cytometry analyzer (bd biosciences; san jose, ca) with bd facs diva and data were analyzed with flowjo software (tree star; ashland, or). gating strategies are shown in supplementary fig. ., to assess the effect of t cell-derived cytokines on macrophage differentiation, monocytes were harvested […]

PMCID: 5958137
PMID: 29773794
DOI: 10.1038/s41467-018-04420-4

[…] washing with pbs (0.3% fbs), the cells were suspended in the same buffer with propidium iodide (1 µg/ml). expression of crt and cd4 was measured on the facsaria ii (bd bioscience) and analyzed by flowjo software (treestar ashland, or)., male balb/c mice (6-week-old) were sensitized with 150 μl of 3% oxa-ethanol solution placed onto the skin of the abdomen at day 0. at day 3, all of the mice […]


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FlowJo review

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Arup Ghosh

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