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FlowJo specifications


Unique identifier OMICS_05624
Name FlowJo
Software type Package/Module
Interface Command line interface
Restrictions to use License purchase required
Operating system Unix/Linux, Mac OS, Windows
License Commercial
Computer skills Advanced
Version 10.2
Stability Stable
Maintained Yes
Wikipedia https://en.wikipedia.org/wiki/FlowJo




No version available



  • person_outline FlowJo Team

FlowJo citations


Enhanced efficacy of combined temozolomide and bromodomain inhibitor therapy for gliomas using targeted nanoparticles

Nat Commun
PMCID: 5959860
PMID: 29777137
DOI: 10.1038/s41467-018-04315-4

[…] A. Cells were washed three times in PBS with 1% BSA. Ten thousand events were recorded and gated for Cy5.5 signal on a FACS Canto II flow cytometer. Percent Cy5.5 positive cells were quantified using FlowJo® software. Data are presented as mean ± SEM from three biological replicates. Statistical significance determined using Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001). […]


Factor XIIIA—expressing inflammatory monocytes promote lung squamous cancer through fibrin cross linking

Nat Commun
PMCID: 5959879
PMID: 29777108
DOI: 10.1038/s41467-018-04355-w

[…] cubated with antibody for 30 min on ice, in the dark. Cells were then washed two times with FACS buffer and taken for flow cytometry analysis on an LSRFortessa. The collected data were analyzed using FlowJo software V10. When performing FACS on lung tissues, Siglec-F positive cells were included with dead cells to remove alveolar macrophages and/or eosinophils. IMs were identified as CD45 + /CD11b […]


MLL fusion driven leukemia requires SETD2 to safeguard genomic integrity

Nat Commun
PMCID: 5959866
PMID: 29777171
DOI: 10.1038/s41467-018-04329-y

[…] harmingen, 553356 = cell, clone 2B8), anti-Mouse Ly-6G (Gr-1) (Biolegend, 108411, clone RB6-8C5). Samples were measured on LSR Fortessa or Canto II flow cytometers (BD Biosciences) and analyzed using FlowJo software (Tree Star). […]


CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema

Nat Commun
PMCID: 5958132
PMID: 29773802
DOI: 10.1038/s41467-018-04418-y

[…] M (#01-2222-42, Affymetrix, Inc.; San Diego, CA). Flow cytometry was performed using a BD Fortessa flow cytometry analyzer (BD Biosciences; San Jose, CA) with BD FACS Diva and data were analyzed with FlowJo software (Tree Star; Ashland, OR). Gating strategies are shown in Supplementary Fig. . […]


Calreticulin and integrin alpha dissociation induces anti inflammatory programming in animal models of inflammatory bowel disease

Nat Commun
PMCID: 5958137
PMID: 29773794
DOI: 10.1038/s41467-018-04420-4

[…] ter washing with PBS (0.3% FBS), the cells were suspended in the same buffer with propidium iodide (1 µg/mL). Expression of CRT and CD4 was measured on the FACSAria II (BD Bioscience) and analyzed by Flowjo software (TreeStar Ashland, OR). […]


Immunogene therapy with fusogenic nanoparticles modulates macrophage response to Staphylococcus aureus

Nat Commun
PMCID: 5958120
PMID: 29773788
DOI: 10.1038/s41467-018-04390-7

[…] st injection, the mice were killed by cardiac perfusion with PBS. The collected lungs were homogenized and the homogenates were processed with the LSR Fortessa FACS instrument, and analyzed using the FlowJo software (FlowJo, LLC). For immunofluoresce microscopy of infected lungs, mice were intravenously injected 24 h post infection with DiI-loaded formulations of NF and fusogenic particles (withou […]


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FlowJo institution(s)

FlowJo review

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Arup Ghosh

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It's an all in one suite for FACS data analysis.