Fluidigm software statistics

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Fluidigm software specifications

Information


Unique identifier OMICS_03948
Name Fluidigm software
Software type Toolkit/Suite
Interface Graphical user interface
Restrictions to use License purchase required
Biological technology Fluidigm Corporation
Operating system Unix/Linux
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes

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Maintainer


  • person_outline Jennifer Hart <>

Fluidigm software in publications

 (8)
PMCID: 5801506
PMID: 29212784
DOI: 10.15252/emmm.201708076

[…] as the number of positive partitions increases, so does the probability that some partitions will contain more than one target molecule (sanders et al, ). the corrected values were provided by the fluidigm software as “estimated targets”. to calculate the number of copies per ng of genomic dna, we used the following equation: no. of copy/ngdna = [no. of est. targets/4.59 (μl/panel)] * [10 (μl […]

PMCID: 5661172
PMID: 29118739
DOI: 10.3389/fmicb.2017.02017

[…] the final community (dna samples from microcosms or batch cultures) relative to their abundances in the initial community (suspension of mixed species used as inoculum), we exported data from the fluidigm software in r to analyze relative species abundance based on the 2-δδct method (figure ) using the following equation:, with δδct = δctifinal-δctiinitial = [cti – ctuniv]final – [cti – […]

PMCID: 5404762
PMID: 28441455
DOI: 10.1371/journal.pone.0176043

[…] ()., rt-qpcr was used to validate selected degs obtained in the iim-gc (n = 7), cim-gc (n = 4) and im-nogc (n = 11) groups (). only 51% of reactions passed the quality control defined by the fluidigm software, probably reflecting rna degradation. of the 19 degs selected from the microarray, 9 were also differentially expressed in the qpcr, but only in 7 of them (36.84% of total degs) […]

PMCID: 4884136
PMID: 27111842
DOI: 10.1038/ncb3346

[…] and custom-designed primer sets were loaded onto a fluidigm 96.96 dynamic array ifc and run on a biomark system (fluidigm) using ssofast sybr green for detection (biorad). data were analysed using fluidigm software and normalized to gusb expression. for analysis of il1a and il1b expression, pools of 100 cells were directly sorted into 2x cellsdirect reaction buffer and pre-amplified […]

PMCID: 4959523
PMID: 27073098
DOI: 10.1128/mBio.02214-15

[…] same gene obtained with different primers are differentiated with a gene suffix (for example, aada and aada.1)., threshold cycle values obtained during library generation were quality checked by the fluidigm software and compared to values obtained previously with other high-throughput qpcr platforms () to access the reliability of the gene quantification. following these quality checks, […]


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