Provides a more quantitative report of spatial distribution of fluorescent molecules, to increase quality of super-resolved images of biological samples and to maintain performance at high imaging density. DAOSTORM uses a fixed-shape model PSF for fitting; an extension using PSFs with variable shape will allow fitting to fluorophores with fixed dipole orientation and combination with three-dimensional STORM methods.
Analyzes, processes and visualizes multi-dimensional microscopy images. BioImageXD puts open-source computer science tools for three-dimensional visualization and analysis into the hands of all researchers, through a user-friendly graphical interface tuned to the needs of biologists. BioImageXD has no restrictive licenses or undisclosed algorithms and enables publication of precise, reproducible and modifiable workflows. It allows simple construction of processing pipelines and should enable biologists to perform challenging analyses of complex processes.
Gathers system calibration and image processing functions. QSIM can determine photons and noise maps for both reconstructed sectioned and wide-field images. It needs camera gain measurement and image contrast modulation measurement to be applied in quantitative structured illumination microscopy (SIM) image processing. This tool aims to simplifies the conversion of SIM from being a qualitative imaging technique into a confocal-like, quantitative imaging modality.
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