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FlyRNAi specifications


Unique identifier OMICS_11006
Name FlyRNAi
Restrictions to use None
Database management system MySQL
Community driven No
Data access Browse
User data submission Not allowed
Maintained Yes


  • Invertebrates
    • Drosophila melanogaster


  • person_outline Ian Flockhart

Publications for FlyRNAi

FlyRNAi citations


A high throughput, functional screen of human Body Mass Index GWAS loci using tissue specific RNAi Drosophila melanogaster crosses

PLoS Genet
PMCID: 5897035
PMID: 29608557
DOI: 10.1371/journal.pgen.1007222

[…] gene symbols (, we identified the closest Drosophila orthologs to each of these human genes according to DIOPT—DRSC Integrative Ortholog Prediction Tool ([], as well as pairwise alignments generated using BLAST of NCBI (for example: In Step 4, we obtained available RNAi knockdown li […]


Integrated biology approach reveals molecular and pathological interactions among Alzheimer’s Aβ42, Tau, TREM2, and TYROBP in Drosophila models

Genome Med
PMCID: 5875009
PMID: 29598827
DOI: 10.1186/s13073-018-0530-9

[…] ic process, nucleus, organelle part, and macromolecular complex, which account for 14.6%, 12.2%, 10%, 8.8%, and 7.2% of the 10,938 high confidence human orthologous genes (DIOPT score > 1; accordingly, with FDR adjusted FET p value < 1.2E-58. In contrast, the immune system genes only account for < 1.1% of the orthologs and were not enriched (FET p […]


RNA Interference (RNAi) Screening in Drosophila

PMCID: 5844339
PMID: 29487145
DOI: 10.1534/genetics.117.300077

[…] hila Stock Center (BDSC), and the National Institute of Genetics (NIG) distribute different libraries to researchers worldwide (; ; ; ; ). These resources and further information can be found at:,,,, and These collections and all associated information can also be se […]


Tools Allowing Independent Visualization and Genetic Manipulation of Drosophila melanogaster Macrophages and Surrounding Tissues

PMCID: 5844306
PMID: 29321168
DOI: 10.1534/g3.117.300452

[…] : 5′-TCCACCTATCGCATTAAACACC-3′ and 5′-ACAGTCCCTCACAATACGGTT-3′.RpL32: 5′-AGCATACAGGCCCAAGATCG-3′ and 5′-TGTTGTCGATACCCTTGGGC-3′.These four sets of primers were obtained from FlyPrimerBank ( qPCR was run on a LightCycler 480 (Roche) and data were analyzed using LightCycler 480 Software and Prism.mCherry: 5′-ACATCCCCGACTACTTGAAGC-3′ and 5′ ACCTTGTAGATGAACTCGCCG- […]


A gene specific T2A GAL4 library for Drosophila

PMCID: 5898912
PMID: 29565247
DOI: 10.7554/eLife.35574.023

[…] Gold collection and prioritized the genes if their human ortholog(s) are disease-related (). We also prioritized genes based on the nomination from scientific community through a web site ( sgRNA targeting the qualified CDS introns were selected based on their efficiency score and specificity annotated at Find CRISPR Tool (). The homology arms upstream or […]


A population of adult satellite like cells in Drosophila is maintained through a switch in RNA isoforms

PMCID: 5919756
PMID: 29629869
DOI: 10.7554/eLife.35954.020
call_split See protocol

[…] performed according to (). For generating guide RNAs, two protospacers were selected (sgRNA1 GCATTCCGCAGGTTTAGTCAC and sgRNA2 GCGATAACCCGGCGACCTCC) flanking 5’ and 3’ Enhancer-3 regions, ( The protospacers were cloned into the tandem guide RNA expression vector pCFD4 (Addgene #49411) ( […]


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FlyRNAi institution(s)
Department of Genetics, Harvard Medical School, Boston, MA, USA; Drosophila RNAi Screening Center and Transgenic RNAi Project, Harvard Medical School, Boston, MA, USA; Marine Science Center, Northeastern University, Nahant, MA, USA; Howard Hughes Medical Institute, Boston, MA, USA
FlyRNAi funding source(s)
This work was supported by NIH NIGMS [R01 GM067761, NIGMS R01 GM084947]; NIH [R24 RR032668, R24 OD021997]; Dana Farber/Harvard Cancer Center [NCI Cancer Center Support Grant # NIH 5 P30 CA06516]; Howard Hughes Medical Institute.

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