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FoCuS-point specifications


Unique identifier OMICS_11021
Name FoCuS-point
Software type Application/Script
Interface Command line interface
Restrictions to use None
Input data FoCuS-point was initially designed to work with ‘.pt3’ files which are the output format from PicoQuant PicoHarp hardware, however correlation and time-gating can be applied to any file which contains TCSPC time-tag data if it is correctly parsed. In addition, FoCuS-point can also take already processed data in a variety of different formats.
Operating system Unix/Linux, Mac OS, Windows
Programming languages Python
Computer skills Advanced
Stability Stable
Maintained Yes


No version available



  • person_outline Dominic Waithe

Publication for FoCuS-point

FoCuS-point citations


A dynamic and adaptive network of cytosolic interactions governs protein export by the T3SS injectisome

Nat Commun
PMCID: 5490264
PMID: 28653671
DOI: 10.1038/ncomms15940

[…] f three measurements was carried out on one bacterium. Obtained FCS curves were fitted with two-dimentional+triplet model to obtain the transit diffusion time and the number of particles, , using the FoCuS-point software. Each fit was evaluated individually; in case the fit quality was not good enough (that is, when χ2>0.05), the according FCS data was excluded. […]


Amiata Donkey Milk Chain: Animal Health Evaluation and Milk Quality

PMCID: 5090121
PMID: 27853717
DOI: 10.4081/ijfs.2016.5951

[…] reach the current number of about 2300 heads, more than double compared to 2005 (http://www. Amiata donkey milk supply chain was recently created in Tuscany, which represents a focus point for the protection of this breed. The creation of an innovative system focusing on donkey milk is particularly interesting for Central European and Mediterranean countries, where native do […]


Avian Influenza A(H5N1) Virus in Egypt

PMCID: 4766899
PMID: 26886164
DOI: 10.3201/eid2203.150593

[…] ultry owners to report outbreaks and providing them appropriate compensation; 5) intervening, when poultry outbreaks are reported, by culling infected poultry and setting monitoring zones around each focus point; 6) properly decontaminating infected farms; 7) encouraging the use of disinfectants in backyards where poultry are raised; 8) increasing awareness about the effects of avian influenza; 9) […]


A straightforward approach for gated STED FCS to investigate lipid membrane dynamics

PMCID: 4641872
PMID: 26123184
DOI: 10.1016/j.ymeth.2015.06.017

[…] k data inspection, but have custom written an FCS data analysis software to quickly facilitate batch generation and analysis of a large number of gated FCS data. The custom written software is called FoCuS – point, and is freely available at The raw fluorescence intensity data was loaded into the software and processed using a variant of a fast TCSP […]


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FoCuS-point institution(s)
Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK; MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK
FoCuS-point funding source(s)
Alfred Benzon Foundation, Medical Research Council (grant ref MC_UU_12010/unit programmes G0902418 and MC_UU_12025), MRC/BBSRC/EPSRC (grant ref MR/K01577X/1), Wolfson Foundation and Wellcome Trust (grant ref 104924/14/Z/14)

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