Fluorescence recovery after photobleaching data analysis software tools
Fluorescence recovery after photobleaching (FRAP) is a fluorescence microscopy technique to measure the kinetic of molecular diffusion though tissue or cells. Usually, a molecule of interest is labeled with a fluorescent probe (or fused with a fluorescent reporter) and then an area is photobleached with a laser to inhibit fluorescence and observe its recovery, a sign of molecule redistribution. This technique is commonly used to study protein mobility, cellular exchanges, and protein binding. FRAP software tools are used for data normalization, visualization, simulation of 3 dimensional models, and more.