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Analyzes, processes and visualizes multi-dimensional microscopy images. BioImageXD puts open-source computer science tools for three-dimensional visualization and analysis into the hands of all researchers, through a user-friendly graphical interface tuned to the needs of biologists. BioImageXD has no restrictive licenses or undisclosed algorithms and enables publication of precise, reproducible and modifiable workflows. It allows simple construction of processing pipelines and should enable biologists to perform challenging analyses of complex processes.
PyFRAP / Python Fluorescence Recovery After Photobleaching
Fits numerical simulations of three-dimensional models to fluorescence recovery after photobleaching (FRAP)/inverse FRAP (iFRAP) data and accounts for bleaching/photoconversion inhomogeneities. PyFRAP is able to apply post-bleach image as initial condition, and numerically simulates the FRAP experiment in realistic two-dimensional or three-dimensional experiment geometries. Moreover, it can determine the diffusion coefficients of fluorescent molecules ranging from 3 to 500 kDa in both artificial and biological contexts.
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