FRAP profiler statistics

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Citations per year

Number of citations per year for the bioinformatics software tool FRAP profiler
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This map represents all the scientific publications referring to FRAP profiler per scientific context
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Protocols

FRAP profiler specifications

Information


Unique identifier OMICS_25919
Name FRAP profiler
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Requirements
ImageJ
Source code URL http://worms.zoology.wisc.edu/ImageJ/FRAP_Profiler.java
Maintained Yes

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FRAP profiler citations

 (12)
library_books

Single point single molecule FRAP distinguishes inner and outer nuclear membrane protein distribution

2016
Nat Commun
PMCID: 5007294
PMID: 27558844
DOI: 10.1038/ncomms12562

[…] e fluorescence reaches the plateau stage. Finally, image-induced photobleaching was corrected by normalizing to the time-course decay of fluorescence in non-bleached areas by using the ImageJ plug-in FRAP Profiler. […]

library_books

Extracellular rigidity sensing by talin isoform–specific mechanical linkages

2015
Nat Cell Biol
PMCID: 4662888
PMID: 26523364
DOI: 10.1038/ncb3268

[…] s were acquired every 20 s for 260–280 s; the fluorescence intensity was recorded between 530–570 nm. Next, fluorescence intensity data were imported into ImageJ and analysed using the ImageJ plugin ‘FRAP profiler’. Only data with comparable initial mean intensities and sufficient initial photo-bleaching were processed in MATLAB (Mathworks). To determine fluorescence recovery, we assumed a reactio […]

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Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation

2015
Nat Commun
PMCID: 4598568
PMID: 26399325
DOI: 10.1038/ncomms9364
call_split See protocol

[…] diameter and only kinetochores that remained visible within this region for the length of the experiment were included in the analysis. Quantification of fluorescence recovery was obtained using the FRAP profiler plugin of ImageJ, which accounts for correction of overall bleaching. Recovery rates for cytoplasmic and kinetochore Bub1 WT, KD and T589A were determined after fitting a single exponent […]

library_books

Glutamylation of Nap1 modulates histone H1 dynamics and chromosome condensation in Xenopus

2015
PMCID: 4411273
PMID: 25897082
DOI: 10.1083/jcb.201412097

[…] photobleach at 100% power for the Argon/488-nm laser was applied to a 1–2-µm-diameter circle on individual metaphase plates or interphase nuclei. Image stacks were aligned and curve fitted using the FRAP Profiler plugin for ImageJ. […]

library_books

Insulin response epigenetic activation of Egr 1 and JunB genes at the nuclear periphery by A type lamin associated pY19 Caveolin 2 in the inner nuclear membrane

2015
Nucleic Acids Res
PMCID: 4381080
PMID: 25753664
DOI: 10.1093/nar/gkv181

[…] I) in the same-sized boxed areas in the NE (see Figure ) was automatically quantified at each time point, background subtracted, and corrected for loss or gain of fluorescence during imaging by using FRAP Profiler Plugin of MBF-ImageJ. The FI was graphically depicted using Microsoft Excel. The mobility rate (%) of Cav-2-PAGFP or Δ47–86-Cav-2-PAGFP from the solid box (photoactivated area at t0 s) t […]

library_books

A novel GSK3 regulated APC:Axin interaction regulates Wnt signaling by driving a catalytic cycle of efficient βcatenin destruction

2015
eLife
PMCID: 4568445
PMID: 26393419
DOI: 10.7554/eLife.08022.027

[…] bleaching was conducted for 8 s with 100% laser. Movies were processed using FRAP analyzer in ImageJ. Bleached area and cell were outlined, background was subtracted and the movie was processed with FRAP profiler. Values were normalized and recovery plateau and standard error were calculated by averaging 10 movies. For t1/2 values were processed in GraphPad (La Jolla CA) using non-linear regressi […]


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