FRAP profiler specifications

Information


Unique identifier OMICS_25919
Name FRAP profiler
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Requirements
ImageJ
Source code URL http://worms.zoology.wisc.edu/ImageJ/FRAP_Profiler.java
Maintained Yes

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FRAP profiler in publications

 (11)
PMCID: 5007294
PMID: 27558844
DOI: 10.1038/ncomms12562

[…] fluorescence reaches the plateau stage. finally, image-induced photobleaching was corrected by normalizing to the time-course decay of fluorescence in non-bleached areas by using the imagej plug-in frap profiler., two complementary approaches have been used to determine the diffusion coefficients for net proteins. first, if single-molecule trajectories of a protein molecule consist of multiple […]

PMCID: 4662888
PMID: 26523364
DOI: 10.1038/ncb3268

[…] were acquired every 20 s for 260–280 s; the fluorescence intensity was recorded between 530–570 nm. next, fluorescence intensity data were imported into imagej and analysed using the imagej plugin ‘frap profiler’. only data with comparable initial mean intensities and sufficient initial photo-bleaching were processed in matlab (mathworks). to determine fluorescence recovery, we assumed […]

PMCID: 4598568
PMID: 26399325
DOI: 10.1038/ncomms9364

[…] diameter and only kinetochores that remained visible within this region for the length of the experiment were included in the analysis. quantification of fluorescence recovery was obtained using the frap profiler plugin of imagej, which accounts for correction of overall bleaching. recovery rates for cytoplasmic and kinetochore bub1 wt, kd and t589a were determined after fitting a single […]

PMCID: 4411273
PMID: 25897082
DOI: 10.1083/jcb.201412097

[…] photobleach at 100% power for the argon/488-nm laser was applied to a 1–2-µm-diameter circle on individual metaphase plates or interphase nuclei. image stacks were aligned and curve fitted using the frap profiler plugin for imagej., fig. s1 shows the distribution of lengths and widths of individual chromosomes in nap1-depleted egg extracts as well as the quantification of chromatin-bound […]

PMCID: 4381080
PMID: 25753664
DOI: 10.1093/nar/gkv181

[…] in the same-sized boxed areas in the ne (see figure ) was automatically quantified at each time point, background subtracted, and corrected for loss or gain of fluorescence during imaging by using frap profiler plugin of mbf-imagej. the fi was graphically depicted using microsoft excel. the mobility rate (%) of cav-2-pagfp or δ47–86-cav-2-pagfp from the solid box (photoactivated area at t0 s) […]


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