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Protocols

FRAPCalc specifications

Information


Unique identifier OMICS_25625
Name FRAPCalc
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux
License GNU General Public License version 3.0
Computer skills Medium
Version 9.0.1
Stability Stable
Requirements
IgorPro
Maintained Yes

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Versioning


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Maintainer


  • person_outline Kota Miura

FRAPCalc citations

 (5)
library_books

A biochemical network controlling basal myosin oscillation

2018
Nat Commun
PMCID: 5865161
PMID: 29572440
DOI: 10.1038/s41467-018-03574-5

[…] change from the average of Myo-II intensities in different cells with a range marked by pink color.To analyze the dynamics of ROCK-GFP, Flw-YFP, and MyoII-GFP, we used the methods proposed from EMBL FrapCalc software for quantitative analysis of frap data (the software is available at https://github.com/framasoft/framacalc). The detailed analysis method has been outlined at Supplementary Informat […]

library_books

Recruitment Kinetics of Tropomyosin Tpm3.1 to Actin Filament Bundles in the Cytoskeleton Is Independent of Actin Filament Kinetics

2016
PLoS One
PMCID: 5158027
PMID: 27977753
DOI: 10.1371/journal.pone.0168203

[…] range of 0 (minimum fluorescence) to 1 (maximum fluorescence). Data from normalized FRAP curves were then fitted with a double exponential equation using the IgorPRO6 software complemented with the K_FRAPcalc version 9 procedure (Kota Miura, EMBL-Heidelberg, Germany) to derive half-times and mobile fractions. Fits obtained using either single or double exponential FRAP recovery model were compared […]

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CPEB4 is regulated during cell cycle by ERK2/Cdk1 mediated phosphorylation and its assembly into liquid like droplets

2016
eLife
PMCID: 5089860
PMID: 27802129
DOI: 10.7554/eLife.19298.028
call_split See protocol

[…] ces were analysed with Fiji (ImageJ) to register and quantify intensity. Exported data was analysed in Igor (Wavemetrics) with a custom FRAP procedure (open source code version by kota miura. (2016). FrapCalc: First github release. Zenodo. 10.5281/zenodo.46873; https://zenodo.org/record/46873). FRAP curves were fitted with a single exponential model and half time and mobile fractions were extracte […]

library_books

MATtrack: A MATLAB Based Quantitative Image Analysis Platform for Investigating Real Time Photo Converted Fluorescent Signals in Live Cells

2015
PLoS One
PMCID: 4616565
PMID: 26485569
DOI: 10.1371/journal.pone.0140209

[…] software enabling the automated analysis of PC datasets []. While software packages are readily available for Fluorescence Recovery After Photobleaching (FRAP) datasets (e.g. Virtual FRAP, easyFRAP, FRAPCalc []), key experimental differences between FRAP and PC protocols (e.g. one color vs. two color time lapse microscopy) make these packages ill-suited for analysis of PC datasets []. In particul […]

library_books

Structure and Biomechanics of the Endothelial Transcellular Circumferential Invasion Array in Tumor Invasion

2014
PLoS One
PMCID: 3933692
PMID: 24587014
DOI: 10.1371/journal.pone.0089758

[…] ure complete recovery following photobleaching. The intensity within the bleached region and a non-bleached stress fiber (as a control) were first measured using Fiji/ImageJ and then plotted with the FRAPCalc program (Dr. Rolf Sara, Åbo Akademi, Turku, Finland) to obtain the mobile fraction, t1/2, and k values. Statistical significance between TCIA and stress fibers was determined using an unpaire […]

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