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Free-D specifications


Unique identifier OMICS_10251
Name Free-D
Software type Package/Module
Interface Graphical user interface
Restrictions to use Academic or non-commercial use
Operating system Unix/Linux, Mac OS, Windows
Computer skills Medium
Version 1.11
Stability Stable
Maintained Yes


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  • person_outline Philippe Andrey <>

Publication for Free-D

Free-D in publications

PMCID: 5943252
PMID: 29743505
DOI: 10.1038/s41598-018-25600-8

[…] streptomycin. cells were then allowed to adhere for 24 hr at 37 °c/5% co2 to reach a confluence of ~80%. on the day of experimentation, they were washed with pbs and then incubated with 100 µl serum-free d-mem (thus reducing well described serum binding effects) containing either 2 µm cpp conjugate (r8-alexa488 or egfp-r8), 5 µg/ml transferrin-alexa 647 (tf-647) or 0.1 mg/ml dextran (10 kda)-alex […]

PMCID: 5944415
PMID: 29756076
DOI: 10.1016/j.heliyon.2018.e00621

[…] μm aap10 was added to the cultures once daily and finally after staining with calcein red-orange. the cells were cultured for 3 days, after which the media were removed and replaced with phenol red free d-mem (gibco/thermo fisher scientific, waltham, ma) containing 5 μm calcein red-orange, am (molecular probes/thermo fisher scientific). staining was performed at +37 °c for 30 minutes […]

PMCID: 5935701
PMID: 29728594
DOI: 10.1038/s41598-018-25521-6

[…] reactive with anti-integrin β1, anti-integrin α3, or mab rm2, cells were pretreated with these mabs for 30 min at room temperature before plating into wells., cells were starved for 14–16 h in serum-free d-mem, and harvested with 0.02% edta in pbs. to reduce basal phosphorylation of signaling molecules, cells were rotated for 30 min at 37 °c using a tube rotator (tr-118, as one, osaka, japan). […]

PMCID: 5908783
PMID: 29432813
DOI: 10.1016/j.bbadis.2018.02.002

[…] (perkinelmer) was used to evaluate collagen over-modification. 2.5 × 104 fibroblasts/cm2 were plated into 6-wells-plate and grown for 24 h. cells were then incubated for 2 h with serum-free d-mem containing 4 mm glutamine, 100 μg/ml penicillin and streptomycin and 100 μg/ml (+)‑sodium l-ascorbate (sigma-aldrich) to stimulate collagen production. for steady state experiments […]

PMCID: 5907601
PMID: 29581220
DOI: 10.1161/JAHA.117.008342

[…] phosphatidylcholine and not choline was used. the in vitro stability of the d6‐choline compound was tested and shown to be very stable (data not shown); it thus appears likely that the free d6‐choline, despite being provided at a greater molar level than isotope–labeled phosphatidylcholine previously, was almost not absorbed by the (small) intestine, limiting tma and subsequent […]

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Free-D institution(s)
Analyse et Modélisation en Imagerie Biologique, Neurobiologie de l’Olfaction et de la Prise Alimentaire, Institut National de la Recherche Agronomique, Domaine de Vilvert, Jouy en Josas, France

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