Fluorescence resonance energy transfert data analysis software tools | Laser scanning microscopy
Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. […] Applications extending from a single molecule to live cells will benefit from multidimensional microscopy techniques, particularly those adapted for optical sectioning and incorporating new algorithms for resolving the component contributions to images of complex molecular systems.
Allows calculation of fluorescence resonance energy transfer (FRET) efficiencies pixel-by-pixel in microscopic images. AccPbFRET is an ImageJ plugin that enables users to correct for bleaching of the donor, for the cross-talk of the acceptor and its photoproduct to the donor channel and for partial acceptor photobleaching. The software also provides automatic registration and semi-automatic analysis of large image sets.