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FLIM-FRET analyzer

Automates processing of intensity-based cell image segmentation. FLIM-FRET analyzer can separate objects of interest from the background to delineate whole cells. It simplifies image segmentation into single cells followed by donor lifetime and donor/acceptor fluorescence intensity quantification. For the software to work, users have to create a FRET collection which associates fluorescent intensity and fluorescence decay image datasets. To create a FRET collection, users import the donor and acceptor fluorescent channels and the donor fluorescence decay curves.


Enables platform-independent analysis of Fluorescence resonance energy transfer (FRET) by acceptor photo-bleaching. FRETcalc implements the FRETTH algorithm, that is based on pixel selection and pixel-to-pixel analysis of the experimental images. The software is applicable for FRET estimation in organelles with non-continuous and continuous shapes, as well as for non-protein fluorophores. The output options include histograms and plots of the donor, acceptor, and FRET intensities in the analyzed region of interests (ROIs).

ExiFRET “exciton FRET”

Calculates fluorescence (or Förster) resonance energy transfer (FRET) between fluorophores distributed in any geometry. ExiFRET extends the Monte Carlo calculation scheme that allows calculation of the efficiency of energy transfer between multiple fluorophores, either in ensembles or attached to single molecules. The software has been used to predict the efficiency for fluorophores randomly distributed in two and three dimensions linked in donor-acceptor pairs with excluded volumes, and linked in pentamers.