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FRETcalc specifications


Unique identifier OMICS_25905
Name FRETcalc
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Computer skills Advanced
Version 5.0
Stability Stable
Maintained Yes


No version available



  • person_outline David Stepensky
  • person_outline David Stepensky

Publication for FRETcalc

FRETcalc citations


Voltage gated sodium channels assemble and gate as dimers

Nat Commun
PMCID: 5727259
PMID: 29233994
DOI: 10.1038/s41467-017-02262-0
call_split See protocol

[…] obtained with an Hamamatsu ORCA-ER charge-coupled device (12 bit) under the control of the SLIDEBOOK software (Intelligent Imaging Innovations, Denver, CO). Images were analyzed with ImageJ using the FRETCALC plugin. FRETc was calculated as FRET – (a×CFP)−(b×YFP) where the crosstalk coefficient are a = 0.29 and b = 0.04. […]


Constitutive Association of Tie1 and Tie2 with Endothelial Integrins is Functionally Modulated by Angiopoietin 1 and Fibronectin

PLoS One
PMCID: 5047623
PMID: 27695111
DOI: 10.1371/journal.pone.0163732
call_split See protocol

[…] hed measurements were collected to control for cell movement or variation of donor intensity levels not induced by acceptor bleaching. Images were background corrected and analyzed using FIJI and the FRETcalc plugin v5.0 to measure changes in donor intensity within regions of interest []. Statistical analysis and graphing was performed in JMP Pro 11. […]


Monomeric gremlin is a novel vascular endothelial growth factor receptor 2 antagonist

PMCID: 5085234
PMID: 27174917
DOI: 10.18632/oncotarget.9286
call_split See protocol

[…] itu control. In all experiments, cells transfected with ECFP-EYFP fusion protein were used as FRET positive controls []. Pixel-pixel FRET efficiency by acceptor photobleaching was also calculated by “FRETcalc v5.0” of ImageJ Software []. […]


ICln: A New Regulator of Non Erythroid 4.1R Localisation and Function

PLoS One
PMCID: 4189953
PMID: 25295618
DOI: 10.1371/journal.pone.0108826

[…] f the cell under examination was bleached. The images were acquired using an HCX PL APO 63x/1.4 OIL objective (Leica Microsystems GmbH) and a scan speed of 700 Hz.FRETeff was then evaluated using the FRETcalc ImageJ plugin as previously reported . […]


PKCε Phosphorylates and Mediates the Cell Membrane Localization of RhoA

PMCID: 3804392
PMID: 24191200
DOI: 10.1155/2013/329063

[…] amorph v7.7 software. Quantitative stoichiometric FRET analysis of the data was performed with proprietary software created by the CLCI staff using MATLAB R2009a (The Mathworks, Natick, MA), and this FRETcalc software can be obtained from the University of Michigan Tech Transfer. The methods and algorithms used in FRET stoichiometry have been previously described [, ]. […]


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FRETcalc institution(s)
Howard Hughes Medical Institute, Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA
FRETcalc funding source(s)
Supported by y the Howard Hughes Medical Institute.

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