FRETcalc statistics

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FRETcalc specifications


Unique identifier OMICS_25905
Name FRETcalc
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Computer skills Advanced
Version 5.0
Stability Stable
Maintained Yes


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  • person_outline David Stepensky <>
  • person_outline David Stepensky <>

Publication for FRETcalc

FRETcalc in publications

PMCID: 5727259
PMID: 29233994
DOI: 10.1038/s41467-017-02262-0

[…] with an hamamatsu orca-er charge-coupled device (12 bit) under the control of the slidebook software (intelligent imaging innovations, denver, co). images were analyzed with imagej using the fretcalc plugin. fretc was calculated as fret – (a×cfp)−(b×yfp) where the crosstalk coefficient are a = 0.29 and b = 0.04., six-well plates of hek293 cells were transfected with polyfect as described […]

PMCID: 5047623
PMID: 27695111
DOI: 10.1371/journal.pone.0163732

[…] measurements were collected to control for cell movement or variation of donor intensity levels not induced by acceptor bleaching. images were background corrected and analyzed using fiji and the fretcalc plugin v5.0 to measure changes in donor intensity within regions of interest []. statistical analysis and graphing was performed in jmp pro 11., 6-well plates were treated with either pbs […]

PMCID: 5085234
PMID: 27174917
DOI: 10.18632/oncotarget.9286

[…] control. in all experiments, cells transfected with ecfp-eyfp fusion protein were used as fret positive controls []. pixel-pixel fret efficiency by acceptor photobleaching was also calculated by “fretcalc v5.0” of imagej software []., huvecs surface biotinylation was performed as previously described []. briefly, serum-starved huvecs were stimulated for 15 minutes at room temperature […]

PMCID: 3804392
PMID: 24191200
DOI: 10.1155/2013/329063

[…] v7.7 software. quantitative stoichiometric fret analysis of the data was performed with proprietary software created by the clci staff using matlab r2009a (the mathworks, natick, ma), and this fretcalc software can be obtained from the university of michigan tech transfer. the methods and algorithms used in fret stoichiometry have been previously described [, ]. , our laboratory reported […]

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FRETcalc institution(s)
Howard Hughes Medical Institute, Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA
FRETcalc funding source(s)
Supported by y the Howard Hughes Medical Institute.

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