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Gap closing software tools | De novo genome sequencing data analysis

Gap closing software tools | De novo genome sequencing data analysis

While next-generation sequencing technologies have made sequencing genomes faster and more affordable, deciphering the complete genome sequence of an organism remains a significant bioinformatics challenge, especially for large genomes. Low sequence coverage, repetitive elements and short read length make de novo genome assembly difficult, often resulting in sequence and/or fragment “gaps” – uncharacterized nucleotide (N) stretches of unknown or estimated lengths. Some of these gaps can be closed by re-processing latent information in the raw reads.

Source text:
(Paulino et al., 2015) Sealer: a scalable gap-closing application for finishing draft genomes. BMC Bioinformatics.

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