GBSA specifications


Unique identifier OMICS_00600
Alternative name Genome Bisulfite Sequencing Analyser
Software type Package/Module
Interface Command line interface, Graphical user interface
Restrictions to use Academic or non-commercial use
Input format BSP, BS-Seeker (text)
Output format Bedgraph, tab-delimited text, images, gene browser
Operating system Unix/Linux, Windows
Programming languages Python
Computer skills Advanced
Stability Stable
Maintained Yes


No version available


  • person_outline Richie Soong

Publication for Genome Bisulfite Sequencing Analyser

GBSA citations


Whole genome DNA methylation: beyond genes silencing

PMCID: 5354935
PMID: 27895318
DOI: 10.18632/oncotarget.13562

[…] ±4 kb) methylation pattern. They reported that DNA methylation level within the ±1 kb region surrounding the TSS showed the greatest correlation with gene repression. Notably, an analysis using GBSA (Genome Bisulfite Sequencing Analyser) [] substantiated this observation. Interestingly, DNA methylation/gene repression correlation was evident at 1 kb downstream of the TSS of the genes. This observa […]


Detection of differentially methylated regions from whole genome bisulfite sequencing data without replicates

Nucleic Acids Res
PMCID: 4666378
PMID: 26184873
DOI: 10.1093/nar/gkv715

[…] significant differences in methylation levels between biological conditions. A number of statistical methods and computational tools have been developed recently, including BSmooth (), MethylKit (), GBSA (), BiSeq (), DSS (), MOABS (), DMAP (), MethylSig () and Bisulfighter ().Similar to other sequencing experiments, the raw data from WGBS experiments are short sequence reads. After alignment and […]

GBSA institution(s)
Cancer Science Institute of Singapore, National University of Singapore, Singapore, Singapore; Department of Pathology, National University of Singapore, Singapore, Singapore

GBSA review

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Anonymous user #224's avatar image No country

Anonymous user #224

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Good features and nice user interface